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SERUM PROTEIN CONCENTRATION OF SHEEP EXPERIMENTALLY POISONED BY Ipomoea asarifolia

Authors: Chaves, Daniel Praseres; Fagliari, José Jurandir; da Silva, Paulo Cesar;

SERUM PROTEIN CONCENTRATION OF SHEEP EXPERIMENTALLY POISONED BY Ipomoea asarifolia

Abstract

In order to evaluate the serum protein concentration of sheep experimentally poisoned by Ipomoea asarifolia, four groups of five animals received diet with inclusion of 25% (G1), 50% (G2), 75% (G3) and 100% (G4) of the plant replacing to Pennisetum purpureum. Blood samples for serum protein concentration evaluation were collected at seven moments (M): before of the I. asarifolia supply (M1), and five (M2), ten (M3), 15 (M4), 20 (M5), 25 (M6) and 30 (M7) days after I. asarifolia supply. The serum concentration of total protein (TP) was measured through spectrophotometry and the protein fractions by means of eletrophoresis in poliacrylamide (SDS-PAGE). Twenty-nine to 31 proteins were detected by densitometric tracing, with molecular weights varying from 19,160 to 250,000 Dáltons (Da). Reduction of the transferrin concentration and increase of acid ?1-glycoprotein level were verified in sheep that ingested 75% and 100% of the plant, immediately before dying. Also, reduction of concentration of IgG heavy chain was evident in M3 and M4, in G3 animals, as well as the level IgG light chain in M2, M3 and M4 of this group, suggesting interference in the immune function in poisoned sheep, especially in those with higher level of the plant on the diet. It is inferred that these proteins can be utilized as auxiliary indicators to diagnose intoxication by I. asarifolia.KEYWORDS: Ipomoea asarifolia; poisonous plants; sheep.

Para avaliar a concentração sérica de proteínas de ovinos experimentalmente intoxicados por Ipomoea asarifolia, quatro grupos de cinco animais receberam dieta com inclusão de 25% (G1), 50% (G2), 75% (G3) e 100% (G4) da planta em substituição ao Pennisetum purpureum. Amostras de sangue para proteinograma foram colhidas em sete momentos (M): antes do início do fornecimento de I. asarifolia (M1), cinco (M2), dez (M3), 15 (M4), 20 (M5), 25 (M6) e 30 (M7) dias após o início do tratamento. A concentração sérica de proteína total (PT) foi obtida por meio de espectrofotometria e as frações protéicas mediante eletroforese em gel de poliacrilamida (SDS-PAGE). Detectou-se 29 a 31 proteínas no traçado densitométrico, com pesos moleculares variando de 19.160 a 250.000 Dáltons (Da). Notou-se redução do teor de transferrina e aumento de ?1-glicoproteína ácida em ovinos que ingeriram 75% e 100% da planta, imediatamente antes de morrerem. Também se constatou diminuição dos teores de IgG de cadeia pesada em M3 e M4 do G3, bem como de IgG de cadeia leve em M2, M3 e M4 desse mesmo grupo, sugerindo interferência na resposta imune dos ovinos intoxicados, especialmente naqueles com maior percentual de inclusão da planta na dieta. Concluiu-se que essas proteínas podem ser utilizadas como indicadores auxiliares no diagnóstico de intoxicação por I. asarifolia.PALAVRAS-CHAVE: Ipomoea asarifolia; ovinos; plantas tóxicas.

Keywords

plantas tóxicas, Ipomoea asarifolia., Toxicology, Ovinos

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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