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Создание ассоциативных симбиотических систем огурца с ризобиями

Создание ассоциативных симбиотических систем огурца с ризобиями

Abstract

Objective. To create and study the propertiesof artificialassociativesymbiotic systems of cucumber withrhizobiapossessingantifungalandgrowth-stimulatingactivity. Materials and methods. The cloning of the gene psl into a plasmidpJN105TurboGFP,electroporationof this designintorhizobia, the inoculationof cucumber plantstransformed by that strainand the evaluation ofnodulation, the determination of antifungalactivity of the strainby dualculture methodand the analysis ofgrowth-promotingeffect ofstrain. Results.The decrease of the numberof hyphaeof fungi inthe roots of plantsinoculated in suspension of rhizobiawas revealed. The growth-promotingeffectof rhizobiaoncucumber plants was found. Conclusions. The treatmentof cucumber plants by the rhizobiapromotes their growthand the protection againstplant pathogens

Цель. Создание и исследование свойств искусственной ассоциативной симбиотической системы огурца обыкновенного с ризобиями, обладающими противогрибковой и ростостимулирующей активностью. Материалы и методы. Клонирование гена psl в плазмиду pJN105TurboGFP, электропорация данной конструкции в ризобии, инокуляция растений огурца трансформированным штаммом и оценка эффективности клубенькообразования, определение противогрибковой активности штамма методом двойной культуры и анализ ростостимулирующего эффекта штамма. Результаты. Было выявлено уменьшение количества гиф грибов на корнях растений, инокулированных в суспензии ризобий. Обнаружен ростостимулирующий эффект ризобий на растения огурца. Заключение. Обработка растений огурца ризобиями способствует их росту и защите от фитопатогенов.

Keywords

РИЗОБИИ, ЛЕКТИН, ПРОТИВОГРИБКОВАЯ И РОСТОСТИМУЛИРУЮЩАЯ АКТИВНОСТЬ

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average