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Диагностическое значение современных методов микробиологической верификации внебольничной пневмонии в клинической практике

Диагностическое значение современных методов микробиологической верификации внебольничной пневмонии в клинической практике

Abstract

The study was carried out to determine etiological structure and informativeness of different methods of detection of agents of community-acquired pneumonia, the sampling included 274 examined patients aged from 16 to 80 years with communityacquired pneumonia of different degree of severity and being under hospital treatment. Besides of standard laboratory and clinical methods of examination of patients with community-acquired pneumonia special techniques of etiological verification were applied: molecular genetic analysis (polymerase chain reaction) of phlegm, qualitative detection of antigen Legionella pneumophila of serogroup I and antigen Streptococcus pneumoniae in samples of urine using quick immune chromatographic test, detection of level of serum specific immunoglobulines class M and G to Chlamidophilia pneumoniae, Mycoplasma pListeria monocytogenes in dynamics using immunoenzyme technique. The etiological structure of community-acquired pneumonia was established based of study results. The analysis of informativeness of different methods of etiological verification of diagnosis of community-acquired pneumonia demonstrated that combination of polymerase chain reaction and serological method is the optimal one.

Цель определение этиологической структуры и информативности различных методов детекции возбудителей внебольничной пневмонии (ВП). Обследованы 274 больных (от 16 до 80 лет) ВП различной степени тяжести, находившихся на стационарном лечении. Наряду со стандартными лабораторными и клиническими методами обследования пациентов с ВП применялись специальные методы этиологической верификации ВП: молекулярно-генетическое исследование (ПЦР) мокроты, качественное выявление антигена Legionella pneumophila серогруппы 1 и антигена Streptococcus pneumoniae в образцах мочи с помощью быстрого иммунохроматографического теста, определение уровня сывороточных специфических иммуноглобулинов классов М и G к Chlamydophila pneumoniae, Mycoplasma pneumoniae, Listeria monocytogenes в динамике иммуноферментным методом. На основании полученных результатов устанавливали этиологическую структуру ВП. Анализ информативности различных методов этиологической верификации диагноза ВП показал, что оптимальным является сочетание ПЦР и серологического метода.

Keywords

ЭТИОЛОГИЯ ВНЕБОЛЬНИЧНОЙ ПНЕВМОНИИ,СЕРОЛОГИЧЕСКИЕ МЕТОДЫ ДИАГНОСТИКИ,ПОЛИМЕРАЗНАЯ ЦЕПНАЯ РЕАКЦИЯ,ETIOLOGY,COMMUNITY-ACQUIRED PNEUMONIA,SEROLOGICAL METHOD OF DIAGNOSTIC,CHAIN REACTION

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
gold