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Питательные среды для культивирования in vitro тканей Ungernia victoris Vved. Ex Artjushenko

Питательные среды для культивирования in vitro тканей Ungernia victoris Vved. Ex Artjushenko

Abstract

Две питательные среды, предложенные ранее для культивирования in vitro тканей редкого лекарственного растения Ungernia victoris, оценены по показателям эффективности прямой регенерации и каллусообразования, а также генетической изменчивости регенерантов, органогенной и неорганогенной культуры ткани в условиях продолжительного культивирования. Установлено, что среда 5С1 пригодна преимущественно для получения каллуса, тогда как среду 5С01 можно эффективно использовать для получения как каллуса, так и регенерантов, а также их пролонгированного культивирования. Обе среды обеспечивают сравнительно низкий уровень генетической изменчивости. Результаты исследования свидетельствуют о высокой эффективности разработанных методик получения культивированных тканей и микроклонального размножения Ungernia victoris Vved. ex Artjushenko.

Two nutrient media proposed earlier to cultivate in vitro rare medicinal herb Ungernia vic toris by the indices of direct regeneration and callusogenesis efficiencies, as well as in terms of genetic variability of the regenerants, organogenic and nonorganogenic culture were assessed under longterm culturing. Medium 5C1 was found to be suitable preferentially for callusogenesis, while medium 5C01 could be used for both callusogenesis and plant regeneration as well as for their longterm cultivation. Both media ensure relatively low level of genetic variability. The obtained results give the possibility to suggest the high efficiency of the developed methods for establishment of tissue culture and micropropagation of Ungernia victoris Vved. ex Artjushenko.

Keywords

МіКРОКЛОНАЛЬНЕ РОЗМНОЖЕННЯ, КУЛЬТУРА ТКАНИН IN VITRO, СОМАКЛОНАЛЬНА МіНЛИВіСТЬ, МИКРОКЛОНАЛЬНОЕ РАЗМНОЖЕНИЕ, КУЛЬТУРА ТКАНЕЙ IN VITRO, СОМАКЛОНАЛЬНАЯ ИЗМЕНЧИВОСТЬ

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
gold