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Формирование рецепторного слоя пьезокварцевого иммуносенсора для определения следовых концентраций рактопамина

Формирование рецепторного слоя пьезокварцевого иммуносенсора для определения следовых концентраций рактопамина

Abstract

The conditions of the nanostructured affine layer's formation on the surface of the gold electrode of the piezoelectric gravimetric sensor intended for highly sensitive and selective detection of trace concentration of beta-agonist (ractopamine) in liquid environments have been experimentally studied and theoretically proved. The conditions of creating of a substrate on the basis of the self-organized monolayers of thiols, silanes have been studied. The conjugates of ractopamine with protein molecules were immobilized on the surface of substrate by the activation with bifunctional reagents. The way of modification of a gold electrode's surface of the piezoelectric sensor was suggested in consideration of the value of concentration sensitivity, weight and stability of the recognizing layer. This way makes it possible to detect the presence of ractopamine in test sample at the level of 0,05 ng/ml and to carry out its definition in the range of 0,1 1 ng/ml.

Экспериментально изучены и теоретически обоснованы условия формирования наноструктурированного аффинного слоя на поверхности золотого электрода пьезокварцевого гравиметрического сенсора, предназначенного для высокочувствительного и селективного определения в жидких средах следовых концентраций бэта-агониста – рактопамина. Изучены условия получения подложки на основе самоорганизованных монослоев тиолов и силанов, на поверхность которой с помощью бифункциональных реагентов иммобилизованы конъюгаты рактопамина с белковыми молекулами. С учетом величины концентрационной чувствительности, массы и устойчивости распознающего слоя предложен способ модифицирования поверхности золотого электрода пьезокварцевого сенсора, позволяющий распознавать присутствие рактопамина в пробе на уровне 0,05 нг/мл и осуществлять его определение в диапазоне 0,1 -1 нг/мл.

Keywords

РАКТОПАМИН, КОНКУРЕНТНЫЙ ФОРМАТ ИММУНОАНАЛИЗА, ИММОБИЛИЗАЦИЯ, БИОРЕЦЕПТОРНЫЙ СЛОЙ, ПЬЕЗОКВАРЦЕВЫЙ ИММУНОСЕНСОР

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
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