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Проблемы лабораторной диагностики риккетсиозов группы клещевой пятнистой лихорадки в России

Проблемы лабораторной диагностики риккетсиозов группы клещевой пятнистой лихорадки в России

Abstract

The article presents analysis of modern techniques of laboratory diagnostic of rickettsiosis of spotted tick-bite fever group. Owing to drastic shortage of list of produced preparations and increasing of specter of detected types of rickettsia in Russia the new approaches to laboratory verification of diagnoses are needed. To detect antibodies to rickettsia of spotted tick-bite fever group can be recommended such techniques as reaction of indirect immune fluorescence and immune enzyme assay with antigens of corresponding types of rickettsia. The most acceptable techniques for detecting and identifying rickettsia of spotted tick-bite fever group are polymerase chain reaction restricting analysis and polymerase chain reaction sequence analysis. The biological methods of analysis are needed to study pathogenic types of rickettsia.

Представлен анализ современных методов лабораторной диагностики риккетсиозов группы клещевой пятнистой лихорадки (КПЛ). В связи с резким сокращением номенклатуры выпускаемых препаратов и увеличением спектра выявленных на территории России видов риккетсий необходимы новые подходы к лабораторной верификации диагнозов. Для выявления антител к риккетсиям группы КПЛ могут быть рекомендованы реакция непрямой иммунофлюоресценции и иммуноферментный анализ с антигенами соответствующих видов риккетсий. Для выявления и идентификации риккетсий группы КПЛ наиболее приемлемы ПЦР-рестрикционный анализ и ПЦР-секвенирование, для изучения патогенных видов риккетсий необходимы биологические методы исследования.

Keywords

РИККЕТСИИ И РИККЕТСИОЗЫ, ЛАБОРАТОРНАЯ ДИАГНОСТИКА

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    influence
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
gold