The effects of pulse-periodic microwave radiation with a pulse repetition frequency in the range of 8-22 per second and intensity of exposure from 70 to 1500 W/cm2 per pulse on the functional activity of isolated liver mitochondria of mice were investigated. The rate of oxygen consumption in different metabolic states of mitochondria by Chance was used as a criterion for evaluating. The degree of conjugation of oxidation and phosphorylation was determined by the ratio of oxygen consumption rate in the phosphorylated and unphosphorylated states. It is shown that after irradiation changed the intensity of respiration on exogenous substrate and with the addition of ADP in the direction of decreasing and increasing (in most cases). The degree of conjugation of oxidation and phosphorylation either remained unchanged or decreased after irradiation by microwave pulses of lower intensities and the pulse repetition frequency from the used. One reason for the increase in oxygen consumption by mitochondria in constant or reduced conjugation of respiration and phosphorylation states could be the utilization of absorbed oxygen for the production of reactive oxygen species by the complexes of the respiratory chain. Reduction of mitochondrial respiration could be caused by the opening in the inner mitochondrial membrane pores nonspecific permeability by the high concentrations of oxidants. That event leads to metabolism of adenine nucleotides and decreasing of the ATP production and oxygen consumption. In the case when the generation rate ROS exceed their recycling, the products of oxidative modification can inhibit components of the antioxidant system and lead to changes in the respiratory chain. It can be assumed the existence of the dual action of reactive oxygen species on the functioning of the respiratory chain because the effect of pulse-periodic microwave radiation on the functional state of mitochondria is a complex dependence on the intensity and frequency of repetition. On the one hand, 2O is produced in the process of electron transfer in the respiratory chain and ATP synthesis, on the other hand, its highest level in mitochondria breaks electron transport and oxidative phosphorylation.

Исследовано влияние импульсно-периодического микроволнового излучения с частотой повторения импульсов в диапазоне 8-22 имп./с и интенсивностью воздействия от 70 до 1 500 Вт/см2 в импульсе на функциональную активность изолированных митохондрий печени мышей по скорости потребления кислорода в различных метаболических состояниях по Чансу. Показано, что однократное воздействие 4 000 импульсов сопровождалось изменением скорости потребления кислорода в фосфорилирующем и нефосфорилирующем состояниях и снижением степени сопряжения процессов окисления и фосфорилирования. Результат зависел от частоты повторения импульсов и интенсивности воздействия. Рассмотрены возможные механизмы влияния микроволновых импульсов на функциональное состояние митохондрий.