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Поиск продуцентов полифенолов и некоторых пигментов среди базидиомицетов

Поиск продуцентов полифенолов и некоторых пигментов среди базидиомицетов

Abstract

General content of polyphenols, carotenoids and melanin in basidiomycetes carpophorus was determined. 50 species were studied, 27 of which belong to the Polyporales form and 23 are to the Agaricales form. In order to determine the total content of phenolic substances spectrophotometric methods were used. Polyphenols were studied in alcoholic extracts through the modified Folin-Chokalteu procedure; melanin — by alkaline hydrolysis and calculated using a calibration curve (by pyrocatechol), carotenoids were studied in acetone extracts and calculated by the Vetshteyn formula. Statistical and cluster analysis of the data enabled to identify species of basidiomycetes that are perspective for biotechnology. The most promising in terms of total polyphenols, carotenoids and melanins of poliporal basidiomycetes are species Fomes fomentarius, Ganoderma applanatum, Ganoderma lucidum and Laetiporus sulphureus , and among agarikal fungi — Fistulina hepatica, Flammulina velutipes, Pleurotus ostreatus, Stropharia rugosoannulata, Agrocybe cylindracea and Tricholoma flavovirens. These species of Basidiomycetes were isolated in pure mycelial culture to find out their biosynthetic activity.

Определяли общее содержание полифенолов, каротиноидов и меланинов в карпофорах базидиомицетов. Исследовано 50 видов, из которых 27 относятся к порядку Polyporales и 23 — Agaricales . С целью определения общего содержания веществ фенольной природы применяли спектрофотометрические методы: полифенолов – в спиртовых вытяжках по модифицированной методике Фолина–Чокальтеу; меланинов — щелочным гидролизом и расчетом по калибровочной кривой (по пирокатехину), каротиноидов — в ацетоновых вытяжках и по формуле Ветштейна. Статистический и кластерный анализы полученных данных позволили выделить перспективные для биотехнологического использования виды базидиомицетов. Максимальное общее содержание полифенолов, каротиноидов и мела ни нов среди полипоральних базидиомицетов обнаружено у видов Fomes fomentarius, Ganoderma applanatum, Ganoderma lucidum и Laetiporus sulphureus , агарикальних – Fistulina hepatica, Flammulina velutipes, Pleurotus ostreatus, Stropharia rugosoannulata, Agrocybe cylindracea и Tricholoma flavovirens. Указанные виды базидиомицетов выделены в чистую мицелиальную культуру для изучения их биосинтетической активности.

Keywords

579.222: 547.979.8, БАЗИДіОМіЦЕТИ, КАРПОФОРИ, ПОЛіФЕНОЛИ, КАРОТИНОїДИ, МЕЛАНіНИ, БАЗИДИОМИЦЕТЫ, КАРПОФОРЫ, ПОЛИФЕНОЛЫ, КАРОТИНОИДЫ, МЕЛАНИНЫ

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
gold