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Выделение, фракционирование и анализ экзополисахаридов Bacillus mucilaginosus

Выделение, фракционирование и анализ экзополисахаридов Bacillus mucilaginosus

Abstract

The purpose of the given stage of researches consists in allocation, clearing, fractionation and studying exopolysaccharides of Bacillus mucilaginosus. Cultivation carried out on a synthetic nutrient medium. Methods of gel-chromatography, ion-exchange chromatography, definition carboxylic groups on Wilson and IR-spectrophotometry were used for analysis. Researched polysaccharide has complex composition and the big molecular weight, it contains two fractions: F1 and F2. Results of defi-nition carboxylic groups on Wilson and IR-spectrophotometry confirm presence carboxylic groups in fraction F2

Цель данного этапа исследований заключалась в выделении, очистке, фракционировании и изучении экзополисахаридов Bacillus mucilaginosus. Культивирование проводили на синтетической питательной среде. Для анализа применяли гель-хроматографию, ионообменную хроматографию, метод определения карбоксильных групп по Вильсон и ИКспекрофотометрию. Исследуемый полисахарид имеет сложный состав и большую молекулярную массу, он содержит две фракции: F1 и F2. Результаты определения карбоксильных групп по Вильсон и ИКспекрофотометрии подтвержда-ют присутствие карбоксильных групп во фракции F2.

Keywords

БАКТЕРИИ BACILLUS MUCILAGINOSUS, ЭКЗОПОЛИСАХАРИД, ВЫДЕЛЕНИЕ, ФРАКЦИОНИРОВАНИЕ, ХРОМАТОГРАФИЯ, ИК-СПЕКТРОФОТОМЕТРИЯ

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average