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Bulletin of KSAU
Article . 2013
Data sources: CyberLeninka - Russian open access scientific library
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Влияние блокатора потенциал-зависимых Са 2+-каналов (верапамил) на биосинтез резвератрола и экспрессию генов CDPK в культуре клеток винограда амурского Vitis amurensis Rupr

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 2013 Russian Publisher:Федеральное государственное образовательное учреждение высшего образования "Красноярский государственный аграрный университет"Journal:Вестник Красноярского государственного аграрного университета (issn: 1819-4036, Copyright policy )

Влияние блокатора потенциал-зависимых Са 2+-каналов (верапамил) на биосинтез резвератрола и экспрессию генов CDPK в культуре клеток винограда амурского Vitis amurensis Rupr

Abstract

The effect of voltage-dependent CA 2+-channels blocker verapamil (VER) on the resveratrol production and the expression of calcium-dependent protein kinases (CDPK) genes in cell culture of Amur grape (Vitis amurensis Rupr.) is shown in the article. Treatment with the VER at a 0.75 mM concentration significantly decreased production of resveratrol and increased expression of additional forms of CDPK genes in grape cell culture with enhanced resveratrol accumulation. The received results prove that CDPK take part in the resveratrol biosynthesis regulation in the grape cells.

В данной работе был показан эффект блокатора потенциал-зависимых Са 2+-каналов верапамила (VER) на продукцию резвератрола и экспрессию генов кальций-зависимых протеинкиназ (CDPK) в культуре клеток Vitis amurensis Rupr. При добавлении в питательные среды 0.75 мМ VER наблюдается достоверное снижение продукции резвератрола и увеличение экспрессии дополнительных форм CDPK в клетках винограда с повышенным содержанием резвератрола. Полученные результаты свидетельствуют о том, что CDPK участвуют в регуляции биосинтеза резвератрола в клетках винограда.

Keywords

КАЛЬЦИЙ-ЗАВИСИМЫЕ ПРОТЕИНКИНАЗЫ, РЕЗВЕРАТРОЛ, ВЕРАПАМИЛ, КУЛЬТУРА КЛЕТОК, ВИНОГРАД АМУРСКИЙ (VITIS AMURENSIS RUPR.), AMUR GRAPE (VITIS AMURENSIS RUPR.)

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
bronze