Powered by OpenAIRE graph
Found an issue? Give us feedback

Способы повышения криоустойчивости спермы хряков-производителей

Способы повышения криоустойчивости спермы хряков-производителей

Abstract

The aim was to study the effect of the introduction of the krezatsina environment for freezing boar semen on her krioustoychivost. Preparing a concentrated sperm to freezing by the method of dialysis, which provides for the introduction of cryoprotective substances lactosecitrate chelate trisovoy (LGHTST) environment through a semipermeable membrane of the sperm without increasing its volume. Dialysis was carried out in spetsustroystve composed of 3 chambers separated by a semipermeable membrane. The inner chamber placed sperm, and two outer filled environment in the ratio 1:2 sperm Wednesday. As an antioxidant krezatsin administered at a dose of 0.15, 0.3, 0.45 and 0.6 g per 1 liter of medium. Studies have found that with the introduction of the environmental indicators krezatsina experimental group exceeded the control on motility at 12.5, 21.9, 28.1 and 31.2%, respectively, of the survival of spermatozoa of40.7, 59, 2, 83.3 and 83.3%, and the preservation of acrosome was higher by 4.0, 9.0, 15.0 and 15.0%, respectively. Krezatsina optimal dose for the introduction of the medium is 0.45 g per 1 liter of medium.

Исследования проводили с целью изучения влияния включения крезацина в состав среды для замораживания спермы хряков на ее криоустойчивость. Концентрированную сперму готовили к замораживанию методом диализа, который предусматривает введение криопротекторных веществ лактозо-хелато-цитратно-трисовой(ЛГХЦТ) среды через полупроницаемую мембрану в сперму без увеличения ее объема. Диализ проводили в спецустройстве, состоящем из 3-х камер, разделенных полупроницаемыми мембранами. Во внутреннюю камеру помещали сперму, а две наружные заполняли средой в соотношении сперма: среда -1:2. В качестве антиоксиданта вводили крезацин в дозе 0,15; 0,3; 0,45 и 0,6 г/л среды. Использование крезацина обеспечило увеличение подвижности спермиев, по сравнению с контролем, на 12,5; 21,9; 28,1 и 31,2 % соответственно, выживаемости спермиев на 40,7; 59,2; 83,3 и 83,3 %, сохранности акросом на 4,0; 9,0; 15,0 и 15,0 %. Оптимальная доза крезацинадля введения в состав среды для криоконсервации спермы хряков 0,45 г/л.

Keywords

КРЕЗАЦИН, ОПЛОДОТВОРЕНИЕ, КАЧЕСТВО СПЕРМЫ, КРИОКОНСЕРВАЦИЯ СПЕРМЫ

  • BIP!
    Impact byBIP!
    selected citations
    These citations are derived from selected sources.
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    0
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    Average
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    Average
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    Average
Powered by OpenAIRE graph
Found an issue? Give us feedback
selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
gold