Today listeriosis is a serious problem not only as a classical nosological unit, but primarily as a food-borne infection. The main means of indication and identification of Listeria in Russia are bacteriological methods that require significant labor and time costs. One of the ways of overcoming these difficulties is to develop and use systems based on the methods for the identification of the genome of the pathogen with the use of polymerase chain reaction (PCR). We set ourselves the goal of developing a system of molecular genetic detection of bacteria species L.monocytogenes, L.ivanovii based on multiplex PCR in real time mode. After selection and design of relevant oligonucleotides and fluorescent probes, we have conducted studies that have proven that the use as a target of the gene fragment prfA allows identification of Lmonocytogenes, L.ivanovii and differentiation of species L.innocua, L.grayi, L.murrayi. The proposed primers and fluorescent probes with dyes R6G and Fam for L.monocytogenes and L.ivanovii are specific to bacteria of these species. Multiplex PCR format in «real time» allows simultaneous amplification and detection of Lmonocytogenes and L.ivanovii in one test tube when using dual channel thermal cyclers (Fam and Hex).

Статья посвящена вопросу разработки мультиплексной ПЦР в режиме «реального времени» для генетической дифференциации патогенных видов листерий, являющихся причиной пищевого листериоза. Авторами показано, что использование праймеров и флуоресцентных зондов с красителями R6G и Fam, фланкирующих фрагмент гена prfA позволяет проводить детекцию бактерий видов Listeria monocytogenes и Listeria ivanovii.