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Амперометрические биосенсоры для определения охратоксина а

Амперометрические биосенсоры для определения охратоксина а

Abstract

Предложены новые амперометрические биосенсоры на основе планарных платиновых электродов, модифицированных многослойными углеродными нанотрубками (МУНТ), и иммобилизованных ферментов: цистеиндесульфгидразы, щелочной фосфатазы и холинэстеразы для определения охратоксина А. Действие биосенсоров основано на ингибирующей способности охратоксина А. Сопоставлены аналитические возможности биосенсоров на основе модифицированных и немодифицированных электродов. Область концентраций определения охратоксина А составляет 1·10 –5 ÷ 1·10 –11 моль/л. Использование МУНТ для модификация электродов обеспечивает расширение области определяемых концентраций, позволяет наблюдать хорошо выраженный эффект ингибирования и более низкие пределы определения. Показана возможность определения охратоксина А в продуктах питания на уровне значений ПДК и ниже.

Novel amperometric biosensors based on platinum screen-printed electrodes modified with multi-walled carbon nanotubes (MWCNTs), and immobilized enzymes (cysteine desulfhydrase, alkaline phosphatase, and cholinesterase) are developed for the determination of ochratoxin A. The action of the biosensors is based on the inhibiting ability of ochratoxin A. The analytical properties of biosensors based on pristine electrodes and electrodes modified with MWCNTs are compared. The working concentration range is 1·10 –5 ÷ 1·10 –11 mol/l. Modification of electrodes with MWCNTs allows achieving a wider range of detectable concentrations, lower detection limits, and more pronounced effect of inhibition. The possibility of determination of ochratoxin A in food products at maximum permissible and lower concentrations is shown.

Keywords

ОХРАТОКСИН А, МИКОТОКСИНЫ, АМПЕРОМЕТРИЧЕСКИЙ БИОСЕНСОР, ХОЛИНЭСТЕРАЗА, ЦИСТЕИНДЕСУЛЬФГИДРАЗА, ЩЕЛОЧНАЯ ФОСФАТАЗА, УГЛЕРОДНЫЕ НАНОТРУБКИ, ЗЕРНОВЫЕ КУЛЬТУРЫ

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
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