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Функциональные характеристики и потребительские свойства биосенсоров на основе пленок Лэнгмюра-Блоджетт для определения биологически активных соединений

Функциональные характеристики и потребительские свойства биосенсоров на основе пленок Лэнгмюра-Блоджетт для определения биологически активных соединений

Abstract

Functional characteristics and consumer properties of biosensors on the basis of Langmuir-Blodgett films for definition of biologically active compounds are studied: L-carnitine, cianocobalamine, dihydrotachysterol, pyridoxine, taurine, hydroxyproline, esterase. It is established that duration of definition by the developed biosensors makes from 1,0 to 3,0 minutes, and operational stability – from 60 to 180 days. Tests of efficiency of application of developed biosensors at definition of biologically active compounds are carried out: L-carnitine, cianocobalamine, dihydrotachysterol, pyridoxine, taurine, hydroxyproline, esterase. It is shown that the minimum limit of definition of biologically active compounds by developed biosensors is 0,01 mm, maximum 10,00 mm. Sensitivity of the developed biosensors makes from 20 to 30 mA/M•cm2, an error of measurement of concentration of anolyte – from 2,0 to 5,0 %, reproducibility of a signal – from 2,0 to 2,5%.

Изучены функциональные характеристики и потребительские свойства биосенсоров на основе пленок Ленгмюра-Блоджетт для определения биологически активных соединений: левокарнитина, цианокобаламина, дигидротахистерола, пиридоксина, таурина, гидроксипролина, эстеразы. Установлено, что длительность определения разработанными биосенсорами составляет от 1,0 до 3,0 минут, а операционная стабильность – от 60 до 180 суток. Проведены испытания эффективности применения разрабатываемых биосенсоров при определении биологически активных веществ: левокарнитина, цианокобаламина, дигидротахистерола, пиридоксина, таурина, гидроксипролина, эстеразы. Показано, что минимальным пределом определения биологически активных веществ разработанными биосенсорами является 0,01 мМ, максимальным 10,00 мМ. Чувствительность разработанных биосенсоров составляет от 20 до 30 мА/М•см2, погрешность измерения концентрации аналита – от 2,0 до 5,0%, воспроизводимость сигнала – от 2,0 до 2,5%.

Keywords

БИОСЕНСОР, ПЛЕНКИ ЛЕНГМЮРА-БЛОДЖЕТТ, БИОЛОГИЧЕСКИ АКТИВНЫЕ СОЕДИНЕНИЯ, ПРЕДЕЛ ОПРЕДЕЛЕНИЯ, ЧУВСТВИТЕЛЬНОСТЬ, ПОГРЕШНОСТЬ, ВОСПРОИЗВОДИМОСТЬ

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
gold