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Генетические детерминанты патогенности микросимбионта Enterococcus faecalis в ассоциации с простейшими Вlastocystis hominis in vitro

Генетические детерминанты патогенности микросимбионта Enterococcus faecalis в ассоциации с простейшими Вlastocystis hominis in vitro

Abstract

It is established that nucleotide sequences of the genes which determine synthesis of pathogenicity factors, take place in the studied bacteria with various ratios, and the frequency of detection of the fragments of required genes changes after joint cultivation of enterococci and protozoan blastocystes. The microsymbiont B.hominis has caused an increase in parameters of frequency of occurrence on cpd and cps genes in Е. faecalis after their joint cultivation. An increase in the frequency of occurrence of required amplicons, specific for cpd and cps genes has resulted in formation of enterococci strains with more intense pathogenicity.

Установлено, что нуклеотидные последовательности генов, детерминирующие синтез факторов патогенности, встречаются у изученных бактерий в различных соотношениях, а частота обнаружения фрагментов искомых генов меняется после совместного культивирования энтерококков с простейшими бластоцистами. Микросимбионт B.hominis вызывал увеличение показателей частоты встречаемости генов cpd и cps у E. faecalis после их совместного культивирования. Увеличение показателей частоты встречаемости искомых ампликонов, специфичных генам cpd и cps приводило к формированию штаммов энтерококков, обладающих более выраженной патогенностью.

Keywords

ПОЛИМЕРАЗНАЯ ЦЕРНАЯ РЕАКЦИЯ, ПРАЙМЕРЫ, "ОСТРОВА" ПАТОГЕННОСТИ, АССОЦИАТИВНЫЕ СИМБИОНТЫ, PATHOGENICITY "ISLANDS"

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
bronze