
Polyclonal immunoglobulins G (subclasses 1, 2, 4) from sera of 255 patients and 69 healthy persons were studied by a combined approach using rivanol treatment and affinity chromatography. Enzymatic reactions were carried out according to the methods that we have previously developed and validated for evaluation of abzyme activity in the patients with different disorders. The levels of DNase, proteolytic BAPNA-amidase (benzoylarginine-p-nitroanilide amidase), and superoxyde dismutase abzyme activity in spondyloarthropathies proved to be significantly higher (p = 0.001), as compared with a control group. Catalase activity of immunoglobulines in the disorders studied was compatible to control levels (p > 0.05). Analysis of relations between abzyme activity and clinical and laboratory signs of the diseases has revealed some significant correlations. Prevalence of abzyme DNAse activity is found in the patients with psoriatic arthritis, as compared to reactive arthritis and ankylosing spondilitis (p
Резюме. Использовались поликлональные иммуноглобулины класса G подклассов 1, 2, 4, выделенные из сыворотки 255 пациентов и 69 здоровых лиц комбинированным риванол-аффиннохроматографическим методом. Постановка реакций осуществлялась согласно методикам, разработанным и апробированным нами ранее при оценке абзимной активности у больных с различной патологией. Уровни ДНКазной (дезоксирибонуклеазной), протеолитической БАПНА-амидазной (бензоиларгинин-паранитроанилид-амидазной) и супероксиддисмутазной абзимной активности при спондилоартропатиях оказались достоверно (р 0,05). Анализ взаимоотношений между абзимной активностью и клинико-лабораторными признаками заболеваний обнаружил ряд достоверных взаимосвязей. Установлено преобладание абзимной ДНКазной активности у пациентов с псориатическим артритом по сравнению с реактивным артритом и анкилозирующим спондилитом (р
СПОНДИЛОАРТРОПАТИИ, АБЗИМНАЯ АКТИВНОСТЬ
СПОНДИЛОАРТРОПАТИИ, АБЗИМНАЯ АКТИВНОСТЬ
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