
ETcocoCmAmp в рекомбинантном штамме E. coli DH5α в зависимости от углеводного субстрата.The article presents the results of the cloning of β-lactamase gene in pETcocoCm plasmid possessing the ability to change its copy number depending on the nutrient medium composition. The level of resistance to ampicillin has been defined depending on a carbohydrate substratum in recombinant E. coli DH5α pETcocoCmAmp strain. Correlation has also been revealed between the level of ampicillin resistance, amount of β-lactamase and pETcocoCmAmp plasmid copy number depending on a carbohydrate substratum in recombinant E. coli DH5α strain
В настоящей работе было проведено клонирование гена β-лактамазы в плазмиду pETcocoCm, обладающую способностью изменять свою копийность в зависимости от состава питательной среды. Был определен уровень устойчивости к ампициллину в рекомбинантном штамме E. coli DH5α pETcocoCmAmp в зависимости от углеводного субстрата. Выявлена корреляция между уровнем устойчивости к ампициллину, количеством β-лактамазы и копийностью плазмиды p
КОНТРОЛЬ ЭКСПРЕССИИ, ПЛАЗМИДА, КОПИЙНОСТЬ ПЛАЗМИДЫ, β-ЛАКТАМАЗА, РЕГУЛЯЦИЯ РЕПЛИКАЦИИ, β-LACTAMASE
КОНТРОЛЬ ЭКСПРЕССИИ, ПЛАЗМИДА, КОПИЙНОСТЬ ПЛАЗМИДЫ, β-ЛАКТАМАЗА, РЕГУЛЯЦИЯ РЕПЛИКАЦИИ, β-LACTAMASE
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