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Компьютерная морфометрия сино-виоцитов: диагностические возможности и значение у больных с посттравматическими остеоартритами

Компьютерная морфометрия сино-виоцитов: диагностические возможности и значение у больных с посттравматическими остеоартритами

Abstract

The article deals with the data of computer morphometric analysis ofsynovial cells in 27 patients 35-53 years old with knee joint posttraumatic osteoarthritis stage I-II according Kellgren and Lowrence classification. The proposed technique of image computer analysis made it possible to apply a large specter of cardio-metric studies of pathologic processes in synovia of patients with post-traumatic osteoarthritis. The study results favored the implementation in computer morphometry new indicators characterizing brightness and optical density of image and permitting to evaluate the degree of compactization and amount of DNA in cells nuclei. The synoviocytes were differentiated on types I,II and III on basis of form parameters and histograms of distribution of cells nuclei brightness. The results of computer analysis of kinetics of proliferation of these cells dyed by Feulgen testified that in synovia of patients with post-traumatic osteoarthritis synoviocytes type II were in G1 -period of interphase and synoviocytes type III were in S-period or G2 -period of interphase.

Представлены данные компьютерного морфологического изучения клеток синовия у 27 больных в возрасте 35-53 лет с посттравматическим остеоартритом (ПТОА) коленных суставов I-II стадии по классификации Kellgren и Lowrence. Предложенный метод компьютерного анализа изображения позволил провести широкий спектр кариометрических исследований патологических процессов в синовии у больных с ПТОА, а полученные результаты исследования сделали возможным использование при компьютерной морфометрии новых показателей, характеризующих яркость и оптическую плотность изображения и позволяющих оценить степень компактизации и количество ДНК в ядрах клеток. На основании параметров формы и гистограмм распределения яркостей ядер клеток были дифференцированы синовиоциты I, II и III типов. Результаты исследования кинетики пролиферации при компьютерном анализе этих клеток, окрашенных по Фельгену, свидетельствовали о том, что в синовии у больных с ПТОА синовиоциты II типа находятся в G 1-периоде интерфазы, а синовиоциты III типа в S-периоде или G 2-периоде интерфазы.

Keywords

КЛЕТКИ СИНОВИЯ, КОМПЬЮТЕРНАЯ МОРФОМЕТРИЯ, ПОСТТРАВМАТИЧЕСКИЙ ОСТЕОАРТРИТ

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
gold