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Изменение вирулентных свойств урогенитальных энтерококков в условиях межмикробных взаимоотношений

Изменение вирулентных свойств урогенитальных энтерококков в условиях межмикробных взаимоотношений

Abstract

У 168 обследованных женщин с воспалительными заболеваниями репродуктивной системы выделены штаммы Mycoplasma hominis и Еnterococcus faecalis. Для выявления микроорганизмов и определения их генетических детерминант патогенности применяли молекулярно-генетический метод. Подбор праймеров и температуры отжига осуществляли при использовании пакета программ «Lasergene» (США). В исследовании выявлены изменения встречаемости генетических детерминант патогенности микросимбионта Е. faecalis, выделенного из микробных консорциумов репродуктивного тракта женщин при наличии и отсутствии в них микоплазм. Установлено, что после сокультивирования энтерококков с микоплазмами различной вирулентности у энтерококков возрастает частота встречаемости пар праймеров сylm (токсигенность, цитолизин), cpd (бактерииоциогенность), cps (адгезия и колонизация) по сравнению с показателями, полученными до сокультивирования.

There have been isolated strains Mycoplasma hominis and Enterococcus faecalis at women (n=168) with imflammatory disease of reproductive tract. PCR was used to detect microorganisms and identification of its genetic determinants of pathogenecity. Primer design and annealing temperature were performed using a software package «Lasergene» (USA). The studies have been revealed changes in the occurrence of genetic determinants of pathogenicity microsymbiont E.faecalis isolated from microbial consortium of women’s reproducnive tract in the presence and in the absence of mycoplasm. It has been found out that microsymbiont M. hominis resulted in increase of frequency of occurrence of genes such as cylm (toxigenicity), cpd (bacterial cytogenecity) and cps (adhesion and colonization) among enterococcus after their joint cultivation.

Keywords

ПАТОГЕННЫЙ ПОТЕНЦИАЛ., МИКРОСИМБИОНТЫ, МЕЖМИКРОБНЫЕ ВЗАИМООТНОШЕНИЯ, РЕПРОДУКТИВНЫЙ ТРАКТ, МИКРОБИОЦЕНОЗ

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
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