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Methodology for obtaining complex bioactive substences from plant resources

Authors: Merenkova, S.P.; Yan, Huang; Wu, Siyu; Chzhou, Khenkhen;

Methodology for obtaining complex bioactive substences from plant resources

Abstract

Svetlana P. Merenkova, candidate of Veterinary Sciences, associate Professor of Department of Food and Biotechnology, South Ural State University, Chelyabinsk, Russia, merenkovasp@susu.ru Yan Huang, student, South Ural State University, Chelyabinsk, Russia. Wu Siyu, student, South Ural State University, Chelyabinsk, Russia. Chzhou Khenkhen, student, South Ural State University, Chelyabinsk, Russia. Меренкова Светлана Павловна, кандидат ветеринарных наук, доцент кафедры «Пищевые и биотехнологии», Южно-Уральский государственный университет, Челябинск, Россия, merenkovasp@susu.ru Ян Хуан, студент, Южно-Уральский государственный университет, Челябинск, Россия. У Сию, студент, Южно-Уральский государственный университет, Челябинск, Россия. Чжоу Кхенхен, студент, Южно-Уральский государственный университет, Челябинск, Россия. Natural biologically active substances obtained from various plant sources are highly valued for their low toxicity, wide range of sources and high yield of final components. It has been established that biologically active (antioxidant) properties are characterized by such biochemical substances as flavonoids, polyphenols, vitamins, especially carotenoids, and active polysaccharides. Rosemary contains significant concentrations of biologically active components, the most important of which are essential oils, carnosol, carnosic acid and rosemary acid. As a rule, there is a synergis-tic effect between different types of antioxidants. The antioxidant capacity of bioactive components after mixing can be significantly higher than that of the original antioxidants, and it is more effective, less energy-consuming, safer and less toxic. Rosemary leaf extracts can enhance their antioxidant activity when interacting with natural carotenoids. The purpose of this study was to design and analyze the optimized parameters for obtaining a complex of biologically active substances based on the principles of synergism of carrot vitamins and rosemary polyphenolic compounds. To confirm and comparatively analyze the bioactive properties of the obtained complex extracts, studies of anti-radical activity, content of polyphenolic and flavonoid compounds were performed. The monoextracts based on 70 % ethanol as well as distilled water extract of rosemary were characterized by the highest DPPH activity and significant concentration of polyphenolic substances. The efficiency of complex plant ethanol extracts was established, the highest concentration of bioactive components and antioxidant activity in the range of 86.9–92.5 % was observed. As a result of this study, the feasibility of application of different extraction agents for the isolation of bioactive compounds from rosemary and carrots was scientifically substantiated, and bioactive components with proven antioxidant properties were developed. Натуральные биологически активные вещества, полученные из различных растительных источников, высоко ценятся за их низкую токсичность, широкий спектр источников и высокий выход конечных компонентов. Установлено, что биологически активными (антиоксидантными) свойствами характеризуются такие биохимические вещества, как флавоноиды, полифенолы, витамины, особенно каротиноиды, и активные полисахариды. Розмарин содержит значительные концентрации биологически активных компонентов, наиболее важными из которых являются эфирные масла, карнозол, карнозиновая кислота и розмариновая кислота. Как правило, существует синергетический эффект между различными типами антиоксидантов. Антиоксидантная способность биоактивных компонентов после смешивания может быть значительно выше, чем у исходных антиоксидантов, и это более эффективно, менее энергозатратно, безопаснее и менее токсично. Экстракты листьев розмарина могут усиливать свою антиоксидантную активность при взаимодействии с природными каротиноидами. Целью данного исследования было разработать и проанализировать оп-тимизированные параметры для получения комплекса биологически активных веществ, основанного на принципах синергизма витаминов моркови и полифенольных соединений розмарина. Для подтверждения и сравнительного анализа биологически активных свойств полученных комплексных экстрактов были проведены исследования антирадикальной активности, содержания полифенольных и флавоноидных соединений. Моноэкстракты на основе 70 % этанола, а также водный экстракт розмарина характеризовались самой высокой DPPH активностью и значительной концентрацией полифенольных веществ. Установлена эффективность комплексных растительных этанольных экстрактов, наблюдалась наибольшая концентрация биоактивных компонентов и антиоксидантная активность в диапазоне 86,9–92,5 %. В результате этого исследования была научно обоснована целесообразность применения различных экстрагентов для выделения биологически активных соединений из розмарина и моркови.

Keywords

УДК 543.48, rosemary leaves, листья розмарина, антиоксидантные свойства, antioxidant properties, биологически активные компоненты, полифенолы, bioactive components, synergistic effect, УДК 581.192, extraction, синергетический эффект, экстракция, polyphenols

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
Green