The bachelor thesis deals with diploid numbers of chromosomes, their morphology and chromosome mechanism of sex determination in four classes of the subphylum Myriapoda. Attention is also paid to mutual phylogenetic relationships within the subphylum. A review of the literature shows the degree of examination of cytogenetic characteristics in individual classes, which is still very low, especially in the classes Pauropoda and Symphyla, and within the class Diplopoda there are still a large number of orders where we completely lack this information. Cytogenetic analyzes revealed a total range of chromosome numbers for: Pauropoda 2n = 12 - 27, Symphyla 2n = 11 - 18, Chilopoda 2n = 14 - 54 and Diplopoda 2n = 8 - 30. Within all four classes, there are both morphologically undifferentiated sex chromosomes and XX/XY and XX/X0 systems. In addition, the X1X1X2X2/X1X20 system is mentioned for the order Spirostreptida. Only a small number of species from the orders Glomerida, Calipodida, Julida, Spirostreptida, Lithobiomorpha and from family Scutigerellidae were able to identify the occurrence of NORs. The work also managed to identify some discrepancies between the data of different works, which shows that especially some older studies were based on poor observation of chromosomes in the karyotype. Key...