Ambient air pollution, caused by harmful pollutants, is considered a severe problem for the natural environment and its components. Effective pollution regulation is possible with reliable data from monitoring stations covering the whole country. Those stations are mainly located at the altitude of 2 m above the surface, possessing their well-known horizontal representativeness. However, the knowledge of the vertical representativeness of these monitoring stations is not sufficient, causing problems while determining the relevance of these measurements for studying the effects of pollutants in various altitudes. The gradient of ozone concentration (determining the change of ozone concentration for each 10 m) was computed to determine the vertical profile of tropospheric ozone (the gradient in this work is using the unit [μg.m-3 /10 m]). Data, containing the ozone concentrations, were measured in altitudes of 2, 8, 50 and 230 m and sourced from the National atmospheric observatory Košetice in the time interval from 6.9.2013 to 31.12.2020. The ozone concentrations show a yearly periodicity, with higher values during the summer period (from 1.4. to 30.9.) and lower values during the winter period (from 1.10. to 31.3.). The gradient for layer 230-50 m outlines considerable stability throughout the year...