Ceratitis fasciventris, Ceratitis anonae and Ceratitis rosa are polyphagous agricultural pests originating from African continent. Their behaviour is heavily altered by pheromones. Insect chemical communication channels are species-specific, represents taxonomic and reproduction barriers. Taxonomy of this group (so-called Ceratitis FAR complex) is unclear. Therefore new chemical approaches along with genetic tests for identification of entities within the cryptic species FAR complex are being developed. To study multi-component mixtures of male volatiles originating from the three mentioned fruit fly species, comprehensive two-dimensional gas chromatography with time- of-flight mass spectrometer (GC×GC-TOFMS) was used. A number of compounds were identified, out of which 23 were found to be distributed and shared among the studied species, 11 out of these were present in all three species. Analyses of male pheromone volatiles, using gas chromatography combined with electroantennographic detector (GC-EAD) revealed 4 common compounds with antennal activity shared among three studied species: methyl (E)-hex-3-enoate, 6-methylhept-5-en-2- one, linalool, and methyl (2E,6E)-farnesoate. The species-specific EAD active compounds in C. fasciventris were esters of isomers of hexenoic acid, whereas isomers of...