
El bagazo de cerveza artesanal representa un subproducto agroindustrial generado en grandes volúmenes, con un alto contenido de carbohidratos fermentables y potencial para su valorización biotecnológica. En este contexto, la presente investigación tuvo como objetivo evaluar la cinética fermentativa y la viabilidad celular de Saccharomyces cerevisiae durante la fermentación alcohólica del bagazo de cerveza artesanal para la obtención de bioetanol. El bagazo de cerveza fue sometido a un proceso de pretratamiento e hidrólisis para la liberación de azúcares fermentables, los cuales fueron posteriormente utilizados como sustrato en fermentaciones anaeróbicas controladas. Durante el proceso fermentativo se monitorearon parámetros cinéticos como el consumo de azúcares, la producción de etanol y la actividad metabólica. La viabilidad de la levadura se determinó mediante métodos de tinción y análisis comparativo con controles positivos y negativos. Los resultados permitieron caracterizar el comportamiento fermentativo de S. cerevisiae en un sustrato alternativo, evidenciando una adecuada adaptación celular y una conversión eficiente de los azúcares disponibles hacia etanol. Asimismo, se observó una relación directa entre la viabilidad celular y la eficiencia fermentativa a lo largo del proceso. En conclusión, el bagazo de cerveza artesanal constituye una materia prima viable para la producción de bioetanol, aportando una alternativa sostenible para el aprovechamiento de residuos agroindustriales y el desarrollo de procesos de economía circular.
Brewer’s spent grain is an agro-industrial byproduct generated in large quantities, characterized by a high content of fermentable carbohydrates and significant potential for biotechnological valorization. In this context, the objective of this study was to evaluate the fermentative kinetics and cell viability of Saccharomyces cerevisiae during the alcoholic fermentation of artisanal brewer’s spent grain for bioethanol production. The brewer’s spent grain was subjected to a pretreatment and hydrolysis process to release fermentable sugars, which were subsequently used as substrate in controlled anaerobic fermentations. Fermentative parameters such as sugar consumption, ethanol production, and metabolic activity were monitored throughout the process. Yeast viability was assessed using staining techniques and comparative analysis with positive and negative controls. The results allowed the characterization of the fermentative behavior of S. cerevisiae using an alternative substrate, demonstrating adequate cellular adaptation and efficient conversion of available sugars into ethanol. In addition, a direct relationship between cell viability and fermentative efficiency was observed during the fermentation process. In conclusion, artisanal brewer’s spent grain represents a viable raw material for bioethanol production, contributing to the sustainable use of agro-industrial residues and the development of circular economy strategies
SACCHAROMYCES CEREVISIAE, BAGAZO, VIABILIDAD, FERMENTACIÓN, BIOETANOL, ANAERÓBICA
SACCHAROMYCES CEREVISIAE, BAGAZO, VIABILIDAD, FERMENTACIÓN, BIOETANOL, ANAERÓBICA
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