?????? cpn60-1, ???????????????????? ???????? ???? ???????????????????????? ?????????????????????? ?? Rhizobium leguminosarum, ???????????????? ???????????? ?????? ?????????????????????????????????? ????????????
?????? cpn60-1, ???????????????????? ???????? ???? ???????????????????????? ?????????????????????? ?? Rhizobium leguminosarum, ???????????????? ???????????? ?????? ?????????????????????????????????? ????????????
?? ?????????????????????????? ???????????? ????????-???????????????????????? ???????????????????? ?????????????????? ???????????? ???????????????????? ??????-?????? ?????????????? ???????????? ?? ?????????? ???????????????????????? ?????????? ?????? ?????????????? ?????????????????????? ?? R. leguminosarum bv. viceae . ????????????????, ???? ?????? cpn60-1 ???? ???????? ???????? ???????????????????? ?? ?????????? ?? ????'???????? ?? ???????? ???????????????????? ?????? ?????????????????????????????? ??????????????.
?? ???????????????????????????? ???????????? ????????-???????????????????????????? ???????????????????? ?????????????? ?????????????? ?????????????????? ??????-?????? ?????????????? ???????????? ???? ???????? ???????????????????????? ?????????? ?????? ?????????????? ?????????????????????? ?? R. leguminosarum. ????????????????, ?????? ?????? cpn60-1 ???? ?????????? ???????? ?????????????? ???? ?????????? ?? ?????????? ?? ?????? ?????????????????? ?????? ?????????????????????????????????? ????????????.
Using the method of site specific mutagenesis we have tried to knock-out one of three homologous genes for chaperonins synthesis in Rhizobium leguminosarum bv. viceae. It has been shown that cpn60-1 gene cannot be destroyed due to its essentiality for cell life.
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