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???????????????????????? ?????????????? ?????????? ???????????????????????? ???????????????????? ?????????????????? Solanum tuberosum ?? ???????????????????????????????? ???????????????????? Solanum rickii

???????????????????????? ?????????????? ?????????? ???????????????????????? ???????????????????? ?????????????????? Solanum tuberosum ?? ???????????????????????????????? ???????????????????? Solanum rickii

Abstract

?????????????????? ???????????????????????? ???? ???????????? ?????????????????????? ???????????????????????? Solanum tuberosum (???????????? ???????????????????? ???? ???????????????????) ?? ?????????? nptII ?? ?????????????? ?????? ???? ?????????????????? ???????? ???????????? ?????????????????????????????? ?????????????? Solanum rickii ?? ?????????? aadA. ???? ???????????????????????? ????????????????????, ???? ?????????????? ?????????????????? ?????????????????????? ??????????????????, ?????????????????????????? ???? ????????????????????????, ???????? ???????????????? ???????????????? ??????????????, ???? ???????? ???????? aadA ???? nptII, ?????????????????????? S. rickii ???? S. tuberosum ?? ?????????????????? ?????????????? ??????????. ???? ???????? ???????? ???????????????????????? ???????????????????????? ??????-????????????????.

?????????????????? ???????????????????????? ???? ?????????????? ?????????????????????? ???????????????????????? Solanum tuberosum (???????????? ?????????????????? ?? ????????????????) ?? ?????????? nptII ?? ?????????????? ?????? ?? ?????????????????????? ???????? ?????????? ???????????????????????????????? ???????????????? Solanum rickii ?? ?????????? aadA. ???? ?????????????????????? ??????????, ?????????????????????? ?????????????????????? ??????????????????, ?????????????????????????? ?? ????????????????????????, ???????? ???????????????? ?????????????????? ????????????????, ?????????????? ???????? aadA ?? nptII, ?????????????????????? S. rickii ?? S. tuberosum, ?????????????????? ?????????????? ??????????, ?????? ???????????????????????? ???????????????????????? ??????-????????????????.

The hybrid plants with transformed plastids were regenerated after fusion of mesophyll protoplasts of Solanum rickii with aadA chloroplast selective marker gene and Solanu tuberosum with nptII nuclear selective marker gene. The hybrid callus clones were selected on the medium containing streptomycine, spectin??mycine and kanamycine. The hybrids were identified on the base of PCR analysis of nuclear and plastid DNAs. The analysis have shown that regenerated plants were somatic hybrids containing aadA and nptII genes, Solanum rickii and Solanum tuberosum nuclear and chloroplast DNA.

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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Average
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