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?????????????????? ?????????????????????????????? ???????????????????? ???????????? Saccharomyces cerevisia?? ?????? ?????????????????????????? ?? ?????????????? ?????????????? ???????????????????????????????????? ?? ?????????????????? ?????????????????? ?? ?????????????????????? ???????????????????????? ??????????

?????????????????? ?????????????????????????????? ???????????????????? ???????????? Saccharomyces cerevisia?? ?????? ?????????????????????????? ?? ?????????????? ?????????????? ???????????????????????????????????? ?? ?????????????????? ?????????????????? ?? ?????????????????????? ???????????????????????? ??????????

Abstract

???????????????????? ?????????????????????????????????? ?????????????????????? ?????????????????? ???????????? S. cerevisi????, ???????????????????????????????? ?? ???????????????? ???10% ???????????????????????????????????? ??? 0,135?? NaCl ??? ???????????, ?????????? ?????????????????????????? ???? ???????????????????? 1, 5 ?? 10????/??????. ?????????????? ???????????????? ????????????????- ?????? ?????????????????????? ?????????????????????? ?????????????????? ?????????????? ???????????????? ???????????????????? ?? ???????????????????????????? ?????????????? ???? ?????????? ???????????????????????????? ?????????????????? ?????????????????? ???? ???????????????? ???????????????????? ???????????????????????? ?? ?????????????????? ?? ???????????????????? ???? ?????? ?????????????????????? ???????????????????????????? ?????????? ?? ???????????????????? 10, 20 ?? 30 ????. ?????????????? ?????????????????????????????????? ?????????????????????? ?????????????????????????????? S. cerevisi???? ??????????????????????, ???????? ???????????????????????? ???????????????????? ????????????, ?????????????????????? ?? ???????????????????? ?????? ??????????????????????????, ???? ?????????? ???????????????????????????? ?????????????????????? ???? ???????????????????? 4???8????/??????. ???????????????????????? ???????????????? ?????????????????????????????????? ?????????????????????? ?????????????????????????????? S. cerevisi???? ?????????????????????? ?????? ?????????????????????????? ?? ???????????????????? ?? ?????????????????? 20 ???? ?????? ???????????????? ???????????????????? ?????????????????????????? ?? ???????????????????? 5????/??????.

?????????????????? ???????????????????????????????? ?????????????????? ???????????????????? ???????????? S. cerevisi????, ?????????????????????????? ?? ?????????????? ???10% ???????????????????????????????????? ??? 0,135?? NaCl ??? ???????????, ?????????? ?????????????????????????? ???? ?????????????????????? 1, 5 ???? 10????/????. ?????????????? ???????????????? ?????????????????????? ?????????????????????? ??????????- ?????????? ?????????????????? ?????????????????? ?????????????????? ?????????????????????? ?? ????????????, ???????? ??????????????????????????, ???? ?????????? ?????????????????????????? ?????????????????? ?????????????????? ?????? ?????????????????? ?????????????????????? ?????????????????? ?? ?????????????????? ???? ???????????????? ?????? ?????? ?????????????????????? ???????????????????????? ?????????? ?? ???????????????????? 10, 20 ?? 30 ????. ???????????? ???????????????????????????????? ?????????????????? ?????????????????????????????? S. cerevisi???? ??????????????????????????????, ???????? ???????????? ?????????????????? ????????????, ?????? ?????????????????? ?? ???????????????????? ?????? ??????????????????????????, ???? ?????????? ?????????????????????????? ?????????????????????????? ???? ?????????????????????? 4???8????/????. ?????????????????????? ???????????????? ?????????????????????????????????? ?????????????????? ?????????????????????????????? S. cerevisi???? ?????????????????????? ?????? ?????????????????????????? ?? ???????????????????? ?????????????????? 20 ???? ???? ?????????????????? ?????????????????????? ?????????????????????? ?? ???????????????????? 5????/????.

The colony-forming ability of the yeast fungi S. cerevisiae suspended in 10% (vol. %) dimethyl sulfoxide ??? 0.135 M NaCl ??? water solution after freezing at the rates of 1, 5 and 10??C/min was determined. The method of multi-point thermography demonstrated the dependence of the average cooling rate in a frozen sample on the crystallisation stage of cell suspension on the coolant cooling rate in the cryostat and on the distance from the axles of cylindrical containers with the diameters of 10, 20 and 30 mm. The high colonyforming ability of the microorganisms S. cerevisiae was observed if a considerable amount of cells in a freezing container is cooled at the rates 4???8??C/min on the crystallization stage. The maximal colony-forming ability of the microorganisms S. cerevisiae was achieved if they were frozen in a container with the diameter of 20 mm at the coolant cooling rate of 5??C/min in the cryochamber.

Keywords

?????????????????????????? ?? ?????????????????????????????????? ????????????????????????

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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