Sequence-related amplified polymorphism (SRAP) analysis of ten acelora (Malpighia glabra L.) accessions includes three cultivars introduced from Vietnam was conducted. Three Vietnamese cultivars were distinguished from each other. In addition, they were distinguished from control accessions. Results of SRAP indicate a relationship between, both Vietnamese cultivars, 'Go Cong' and 'Ben Tre', and slight relationship among them and 'Florida Sweet' and 'Hawaiian Queen'. Vietnamese cultivar 'High Vitamin C' was clustered with 'Maunawili' and 'Rehnborg'. The present study demonstrated the usefullness of SRAP analysis for identification of cultivars introduced from Vietnam.