DETECTION AND IDENTIFICATION OF APPLE PROLIFERATION GROUP PHYTOPLASMAS OF FRUIT CROPS
DETECTION AND IDENTIFICATION OF APPLE PROLIFERATION GROUP PHYTOPLASMAS OF FRUIT CROPS
Целью исследования было изучение, выявление и идентификация фитоплазмозов группы Apple proliferation (АР) на территории Российской Федерации и европейских стран. Из 67 исследованных образцов плодовых культур определены фитоплазмы, входящие в группу AP. Все образцы выделенной ДНК анализировали методом «nested»-ПЦР с помощью универсальных праймеров, разработанных для участка 16-23S генов. Показано, что возбудитель заболевания «пролиферация яблони» (AP) Candidatus Phytoplasma mali обнаружен в Испании, возбудитель заболевания «истощение груши» (PD) Candidatus Phytoplasma pyri — в Республике Дагестан, Италии, Испании, возбудитель заболевания «хлоротическое скручивание листьев абрикоса» (ESFY) Candidatus Phytoplasma prunorum — в Чехии. По результатам секвенирования получены и депонированы в Международную генетическую базу данных (NCBI) участки ДНК фитоплазм группы пролиферации яблони.
In this research were studied the Apple proliferation group phytoplasmas (AP) and their diagnostics. Was estimated the distribution AP group phytoplasmas on the territory of the Russian Federation and European countries. All DNA samples were tested by nested-PCR with universal primers designed against the regions of the genes 16-23S. Were tested more than 60 samples of fruit trees, were detected AP group phytoplasmas. Candidatus Phytoplasma mali was detected in samples from Spain. Ca. Phytoplasma pyri was detected in samples from the Republic of Dagestan, Italy and Spain. Ca. Phytoplasma prunorum was detected in the samples from the Czech Republic. Obtained DNA sequences of regions of the apple proliferation group phytoplasmas were deposited into the National Center for Biotechnology Information (NCBI).
apple proliferation, Генбанк NCBI, plant quarantine, фитоплазмы, polymerase chain reaction, phytoplasma, полимеразная цепная реакция, GenBank NCBI, пролиферация яблони, карантин растений
apple proliferation, Генбанк NCBI, plant quarantine, фитоплазмы, polymerase chain reaction, phytoplasma, полимеразная цепная реакция, GenBank NCBI, пролиферация яблони, карантин растений
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