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Comparison of Resistance to Malathion and Fenpiroximate Detected by Dysruptive Selection of the Genotypes of the Two-Spotted Spider Mite

Authors: Ali H.G.I.; Sundukov O.V.; Astarkhanova T.S.;

Comparison of Resistance to Malathion and Fenpiroximate Detected by Dysruptive Selection of the Genotypes of the Two-Spotted Spider Mite

Abstract

Sensitive and resistant to malathion and fenpyroximate the inbred two-spotted spider mite strains were obtained by disruptive selection. Family selection of genotypes of single females offspring was carried out based on mean arithmetic indices of the death rate (%) after treating mites with the diagnostic concentration of the each acaricide. Inheritance of the character of resistance was determined using the mortality rate of families of hybrid females of the F1 progeny and of the analyzing crossing Fa. To compare the biochemical mechanisms of resistance to acaricides related to the chemical classes of OP and METI, the molecular marker of the resistance gene for OP-carboxyl-esterase isozyme E3 was revealed by electrophoresis.

Чувствительные и резистентные к малатиону и к фенпироксимайту линии обыкновенного паутинного клеща были получены дизруптивным отбором при инбредном размножении. Отбор генотипов потомства единичных самок проводили посемейно по среднеарифметическим показателям величины смертности (в %) при обработке клещей диагностической концентрацией каждого из акарицидов. Характер наследования признака резистентности определяли по уровню смертности семей гибридных самок потомства F1 и анализирующего скрещивания Fа. Для сопоставления биохимических механизмов резистентности к акарицидам, относящимся к химическим классам ФОС и МЕТИ, методом электрофореза выявили молекулярный маркер гена резистентности к ФОС - карбоксилэстеразный изозим Е3.

Keywords

fenpyroximate, dysplastic selection, malathion, фенпироксимайт, genotype, selection, селектирование, дизруптивный отбор, обыкновенный паутинный клещ, малатион, признаки резистентности, генотип, signs of resistance, two-spotted spider mite

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
Green