The aim — to study the possibility of fluorescent cholangiography application to assess extrahepatic bile ducts patency.Materials and methods. The results of the fluorescent cholangiography for evaluation of extrahepatic bile ducts patency were analyzed. A video system for fluorescent cholangiography by Karl Storz (Germany) and indocyanine green were used.Results and discussion. The fluorescence cholangiography in laparoscopic cholecystectomy for the technically complicated acute destructive cholecystitis made it possible to detect cystic duct, common hepatic and common bile duct in laparoscopic cholecystectomy for transient choledocholithiasis suspicion to visualize a free passage of bile into the duodenum and did not allow to visualize the passage of bile in extrahepatic bile ducts during open operation for tumor obstruction.Conclusions. The first experience of fluorescence cholangiography in laparoscopic cholecystectomy showed the technical simplicity of the technique, the possibility of a clear visualization of extrahepatic bile ducts. Assume that fluorescence cholangiography can be used to evaluate the bile ducts patency instead of intraoperative cholangiography or other intraoperative audit methods in certain cases. Evaluation of extrahepatic bile ducts patency in a complete tumor block using fluorescence cholangiography is ineffective.