Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of bacterioferritin A fromMycobacterium tuberculosis
Copyright policy )Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of bacterioferritin A fromMycobacterium tuberculosis
Bacterioferritins (Bfrs) comprise a subfamily of the ferritin superfamily of proteins that play an important role in bacterial iron storage and homeostasis. Bacterioferritins differ from ferritins in that they have additional noncovalently bound haem groups. To assess the physiological role of this subfamily of ferritins, a greater understanding of the structural details of bacterioferritins from various sources is required. The gene encoding bacterioferritin A (BfrA) from Mycobacterium tuberculosis was cloned and expressed in Escherichia coli. The recombinant protein product was purified by affinity chromatography on a Strep-Tactin column and crystallized with sodium chloride as a precipitant at pH 8.0 using the vapour-diffusion technique. The crystals diffracted to 2.1 A resolution and belonged to space group P4(2), with unit-cell parameters a = 123.0, b = 123.0, c = 174.6 A.
Crystallization: methods, Recombinant Proteins: isolation & purification, Bacterial Proteins: isolation & purification, Ferritins: isolation & purification, Crystallography, X-Ray, Cytochrome b Group: isolation & purification, Chromatography, Affinity, Cytochrome b Group: genetics, Escherichia coli: genetics, Bacterial Proteins, Escherichia coli, Bacterial Proteins: chemistry, Cloning, Molecular, info:eu-repo/classification/ddc/530, Ferritins: genetics, Recombinant Proteins: metabolism, Mycobacterium tuberculosis: chemistry, bacterioferritin, Crystallography, X-Ray: methods, Ferritins: chemistry, Recombinant Proteins: genetics, Mycobacterium tuberculosis, Cytochrome b Group, Escherichia coli: metabolism, Bacterial Proteins: metabolism, Recombinant Proteins, Ferritins, Cytochrome b Group: chemistry, Ferritins: metabolism, Mycobacterium tuberculosis: growth & development, Crystallization, Cytochrome b Group: metabolism, Bacterial Proteins: genetics
Crystallization: methods, Recombinant Proteins: isolation & purification, Bacterial Proteins: isolation & purification, Ferritins: isolation & purification, Crystallography, X-Ray, Cytochrome b Group: isolation & purification, Chromatography, Affinity, Cytochrome b Group: genetics, Escherichia coli: genetics, Bacterial Proteins, Escherichia coli, Bacterial Proteins: chemistry, Cloning, Molecular, info:eu-repo/classification/ddc/530, Ferritins: genetics, Recombinant Proteins: metabolism, Mycobacterium tuberculosis: chemistry, bacterioferritin, Crystallography, X-Ray: methods, Ferritins: chemistry, Recombinant Proteins: genetics, Mycobacterium tuberculosis, Cytochrome b Group, Escherichia coli: metabolism, Bacterial Proteins: metabolism, Recombinant Proteins, Ferritins, Cytochrome b Group: chemistry, Ferritins: metabolism, Mycobacterium tuberculosis: growth & development, Crystallization, Cytochrome b Group: metabolism, Bacterial Proteins: genetics
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