Dendritic cells generated from patients with androgen‐independent prostate cancer are not impaired in migration and T‐cell stimulation
Copyright policy )Dendritic cells generated from patients with androgen‐independent prostate cancer are not impaired in migration and T‐cell stimulation
AbstractBACKGROUNDDendritic cell (DC)‐based vaccination has been investigated as immunotherapy for several types of cancer. A potential drawback to vaccination with autologous monocyte‐derived DCs (MoDCs) could be that MoDCs from patients are functionally impaired. In case of androgen‐independent prostate cancer (CaP), the cancer itself, diverse prior therapies, and the hormone manipulation may affect the immune system.METHODSMoDCs from patients suffering from androgen‐independent CaP were generated according to a clinically applicable protocol to evaluate the phenotype, maturation capacity, migration, and T‐cell stimulation of these cells compared with those generated from tumor‐free donors.RESULTSMoDCs generated from CaP patients could be fully matured and efficiently migrated towards the chemokine CCL21. They had a strong potency to activate allogeneic CD4+ and CD8+ T‐cells and to present antigens to specific CTL.CONCLUSIONSOur data suggest that MoDCs from patients with androgen‐independent CaP are functionally intact and hence qualify as cellular vaccines for immunotherapy of advanced stage CaP. © 2005 Wiley‐Liss, Inc.
- University of Konstanz Germany
- University of St. Gallen Switzerland
- University of Salford United Kingdom
- Kantonsspital St. Gallen Switzerland
- Biotechnology Institute Thurgau Switzerland
Male, Cells, monocyte-derived dendritic cells (MoDCs), Cell Communication, Dendritic Cells/cytology, Lymphocyte Activation/immunology, CD8-Positive T-Lymphocytes, In Vitro Techniques, Lymphocyte Activation, CD8-Positive T-Lymphocytes/cytology, Prostatic Neoplasms/immunology, Cancer Vaccines, Immunophenotyping, Cell Movement, Cancer Vaccines/immunology, Androgens/metabolism, Humans, Cells, Cultured, Aged, info:eu-repo/classification/ddc/570, Cultured, Prostatic Neoplasms, Dendritic Cells, Middle Aged, prostate cancer, Cell Movement/immunology, Immunotherapy/methods, Androgens, DC phenotype and functions, Cell Communication/immunology, Immunotherapy, cancer vaccine
Male, Cells, monocyte-derived dendritic cells (MoDCs), Cell Communication, Dendritic Cells/cytology, Lymphocyte Activation/immunology, CD8-Positive T-Lymphocytes, In Vitro Techniques, Lymphocyte Activation, CD8-Positive T-Lymphocytes/cytology, Prostatic Neoplasms/immunology, Cancer Vaccines, Immunophenotyping, Cell Movement, Cancer Vaccines/immunology, Androgens/metabolism, Humans, Cells, Cultured, Aged, info:eu-repo/classification/ddc/570, Cultured, Prostatic Neoplasms, Dendritic Cells, Middle Aged, prostate cancer, Cell Movement/immunology, Immunotherapy/methods, Androgens, DC phenotype and functions, Cell Communication/immunology, Immunotherapy, cancer vaccine
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