The influence of acoustic field distribution and temperature variations on the full-aperture diffraction efficiency of non-collinear acousto-optic tunable filters (AOTFs) was investigated based on tellurium dioxide crystals. The strong acoustic anisotropy of the crystal induces non-uniform acoustic energy distribution, limiting the overall diffraction efficiency. To analyze this effect, the acoustic field distribution within a large-aperture AOTF was simulated, and the diffraction efficiency across different aperture regions was evaluated and experimentally validated. The results demonstrate that sound beam contraction and acoustic energy non-uniformity significantly reduce the peak diffraction efficiency and increase the power required to achieve high diffraction efficiency. Additionally, temperature-induced variations in acoustic velocity alter the acoustic field structure, leading to spatially non-uniform changes in diffraction efficiency. Both simulations and experimental measurements confirm that while the overall impact of temperature on full-aperture diffraction efficiency remains relatively small, localized variations are pronounced, highlighting potential inaccuracies in single-beam-based efficiency measurements.