Myocardial Ischemic Subject’s Thymus Fat: A Novel Source of Multipotent Stromal Cells
Copyright policy )Myocardial Ischemic Subject’s Thymus Fat: A Novel Source of Multipotent Stromal Cells
Adipose Tissue Stromal Cells (ASCs) have important clinical applications in the regenerative medicine, cell replacement and gene therapies. Subcutaneous Adipose Tissue (SAT) is the most common source of these cells. The adult human thymus degenerates into adipose tissue (TAT). However, it has never been studied before as a source of stem cells.We performed a comparative characterization of TAT-ASCs and SAT-ASCs from myocardial ischemic subjects (n = 32) according to the age of the subjects.TAT-ASCs and SAT-ASCs showed similar features regarding their adherence, morphology and in their capacity to form CFU-F. Moreover, they have the capacity to differentiate into osteocyte and adipocyte lineages; and they present a surface marker profile corresponding with stem cells derived from AT; CD73+CD90+CD105+CD14-CD19-CD45-HLA-DR. Interestingly, and in opposition to SAT-ASCs, TAT-ASCs have CD14+CD34+CD133+CD45- cells. Moreover, TAT-ASCs from elderly subjects showed higher adipogenic and osteogenic capacities compared to middle aged subjects, indicating that, rather than impairing; aging seems to increase adipogenic and osteogenic capacities of TAT-ASCs.This study describes the human TAT as a source of mesenchymal stem cells, which may have an enormous potential for regenerative medicine.
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Cytological Techniques::Cell Separation::Flow Cytometry, Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::DNA-Binding Proteins::Basic-Leucine Zipper Transcription Factors::Basic Helix-Loop-Helix Leucine Zipper Transcription Factors::Sterol Regulatory Element Binding Proteins::Sterol Regulatory Element Binding Protein 1, adipose tissue stromal cell, subcutaneous fat, HLA DR antigen, Regenerative Medicine, Grasa subcutánea, Medicina regenerativa, Ensayo de unidades formadoras de colonias, adipose tissue cell, CD34 antigen, CD133 antigen, Diferenciación celular, Adipogenesis, Mesenchymal Stromal Cells, Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Differentiation::Adipogenesis, Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Growth Processes::Cell Proliferation, Flow Cytometry, Adipose Tissue, bone development, Medicine, Medical Subject Headings::Anatomy::Tissues::Connective Tissue::Adipose Tissue::Adipose Tissue, White::Subcutaneous Fat, Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression, Sterol Regulatory Element Binding Protein 1, Medical Subject Headings::Diseases::Cardiovascular Diseases::Heart Diseases::Myocardial Ischemia, Science, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Clinical Laboratory Techniques::Immunologic Tests::Immunophenotyping, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Clinical Laboratory Techniques::Cytological Techniques::Colony-Forming Units Assay, Anciano, Thymus adipose tissue, Subcutaneous Fat, Medical Subject Headings::Anatomy::Tissues::Lymphoid Tissue::Thymus Gland, Colony-Forming Units Assay, Humans, Aged, Mediana edad, CD19 antigen, Proliferación de la célula, CD45 antigen, cell adhesion, Mesenchymal Stem Cells, Medical Subject Headings::Phenomena and Processes::Musculoskeletal and Neural Physiological Phenomena::Musculoskeletal Physiological Phenomena::Musculoskeletal Physiological Processes::Musculoskeletal Development::Bone Development::Osteogenesis, Stem cell characterization, Mesenchymal stem cells, Citometría de flujo, cell structure, Myocardial Ischemia, Gene Expression, Adipose tissue stromal cells, thymus, Osteogenesis, Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Middle Aged, endoglin, Reverse Transcriptase Polymerase Chain Reaction, Q, R, Cell Differentiation, Middle Aged, adipose tissue, stroma cell, Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Carrier Proteins::Fatty Acid-Binding Proteins, Isquemia miocárdica, Medical Subject Headings::Disciplines and Occupations::Health Occupations::Medicine::Regenerative Medicine, Research Article, Tejido adiposo, heart muscle ischemia, Subcutaneous adipose tissue, colony forming unit F, osteocyte, thymus adipose tissue, Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Aged, Thymus Gland, adipocyte, Proteínas de unión a ácidos Ggrasos, Fatty Acid-Binding Proteins, adipogenesis, Immunophenotyping, Osteogénesis, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction::Reverse Transcriptase Polymerase Chain Reaction, Células del estroma mesenquimatoso, Proteína 1 de unión a los elementos reguladores de esteroles, Medical Subject Headings::Anatomy::Tissues::Connective Tissue::Adipose Tissue, Cell Proliferation, Reacción en cadena de la polimerasa de transcriptasa inversa, Adipogénesis, Thy 1 antigen, Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Differentiation, CD14 antigen, cell differentiation, Inmunofenotipificación, 5' nucleotidase, Timo, Medical Subject Headings::Anatomy::Cells::Connective Tissue Cells::Stromal Cells::Mesenchymal Stromal Cells, Expresión génica
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Cytological Techniques::Cell Separation::Flow Cytometry, Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::DNA-Binding Proteins::Basic-Leucine Zipper Transcription Factors::Basic Helix-Loop-Helix Leucine Zipper Transcription Factors::Sterol Regulatory Element Binding Proteins::Sterol Regulatory Element Binding Protein 1, adipose tissue stromal cell, subcutaneous fat, HLA DR antigen, Regenerative Medicine, Grasa subcutánea, Medicina regenerativa, Ensayo de unidades formadoras de colonias, adipose tissue cell, CD34 antigen, CD133 antigen, Diferenciación celular, Adipogenesis, Mesenchymal Stromal Cells, Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Differentiation::Adipogenesis, Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Growth Processes::Cell Proliferation, Flow Cytometry, Adipose Tissue, bone development, Medicine, Medical Subject Headings::Anatomy::Tissues::Connective Tissue::Adipose Tissue::Adipose Tissue, White::Subcutaneous Fat, Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression, Sterol Regulatory Element Binding Protein 1, Medical Subject Headings::Diseases::Cardiovascular Diseases::Heart Diseases::Myocardial Ischemia, Science, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Clinical Laboratory Techniques::Immunologic Tests::Immunophenotyping, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Clinical Laboratory Techniques::Cytological Techniques::Colony-Forming Units Assay, Anciano, Thymus adipose tissue, Subcutaneous Fat, Medical Subject Headings::Anatomy::Tissues::Lymphoid Tissue::Thymus Gland, Colony-Forming Units Assay, Humans, Aged, Mediana edad, CD19 antigen, Proliferación de la célula, CD45 antigen, cell adhesion, Mesenchymal Stem Cells, Medical Subject Headings::Phenomena and Processes::Musculoskeletal and Neural Physiological Phenomena::Musculoskeletal Physiological Phenomena::Musculoskeletal Physiological Processes::Musculoskeletal Development::Bone Development::Osteogenesis, Stem cell characterization, Mesenchymal stem cells, Citometría de flujo, cell structure, Myocardial Ischemia, Gene Expression, Adipose tissue stromal cells, thymus, Osteogenesis, Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Middle Aged, endoglin, Reverse Transcriptase Polymerase Chain Reaction, Q, R, Cell Differentiation, Middle Aged, adipose tissue, stroma cell, Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Carrier Proteins::Fatty Acid-Binding Proteins, Isquemia miocárdica, Medical Subject Headings::Disciplines and Occupations::Health Occupations::Medicine::Regenerative Medicine, Research Article, Tejido adiposo, heart muscle ischemia, Subcutaneous adipose tissue, colony forming unit F, osteocyte, thymus adipose tissue, Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Aged, Thymus Gland, adipocyte, Proteínas de unión a ácidos Ggrasos, Fatty Acid-Binding Proteins, adipogenesis, Immunophenotyping, Osteogénesis, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction::Reverse Transcriptase Polymerase Chain Reaction, Células del estroma mesenquimatoso, Proteína 1 de unión a los elementos reguladores de esteroles, Medical Subject Headings::Anatomy::Tissues::Connective Tissue::Adipose Tissue, Cell Proliferation, Reacción en cadena de la polimerasa de transcriptasa inversa, Adipogénesis, Thy 1 antigen, Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Differentiation, CD14 antigen, cell differentiation, Inmunofenotipificación, 5' nucleotidase, Timo, Medical Subject Headings::Anatomy::Cells::Connective Tissue Cells::Stromal Cells::Mesenchymal Stromal Cells, Expresión génica
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