Abstract Background and purpose Technology acceptance has been researched for decades. While some technologies are widely accepted, others are perceived as a threat, such as microchip implants. In this study, a two-step structural equation modeling approach was used to evaluate a new research model on microchip implant acceptance. Methodology A structural equation modeling model was developed to identify what influences the perceived acceptance of microchip implants. To determine differences in attitudes toward microchip implants, the study was conducted in five Eastern European countries. Results The results show that the influence of the factors does not differ significantly across the countries studied. Age, trust, and perceived usefulness affected the overall intention to use microchip implants, while ease of use was significant in only one country. Differences were found in perceptions of the right to privacy and conspiracy theories. The usefulness of microchip implants in pandemic was significant in all countries. Conclusion Small differences in attitudes towards microchip implants suggest that a general model of microchip implant acceptance could be constructed based on the data collected. In addition to these findings, our study noted the lack of legislation for microchip implants in the region and a lack of knowledge about this technology.