Systems biology examines organisms and processes by a holistic approach instead of the traditional reductionist approach. This can be beneficial to researchers and bioengineers as it allows for the formulation of the “big picture” where visualization of the influences and interactions of system components can occur. Significant amounts of data about the system are required, however, for this approach to be successful. Here, the polyhydroxyalkanoate (PHA) homeostasis process in Ralstonia eutropha is examined with a systems biology slant. Copious amounts of data exist about the enzymes, structural proteins and other players that make up the PHA production/mobilization process in R. eutropha. These data are utilized to create an interaction map of the PHA granule (and PHA homeostasis enzymes) that can inform PHA production strain engineering by providing information about key interactions