Secretome profiling of human epithelial cells exposed to cigarette smoke extract and their effect on human lung microvascular endothelial cells
Copyright policy )Secretome profiling of human epithelial cells exposed to cigarette smoke extract and their effect on human lung microvascular endothelial cells
AbstractCigarette smoke (CS) is one of the leading causes of pulmonary diseases and can induce lung secretome alteration. CS exposure-induced damages to human pulmonary epithelial cells and microvascular endothelial cells have been extensively demonstrated; however, the effects of the secretome of lung epithelial cells exposed to CS extracts (CSE) on lung microvascular endothelial cells are not fully understood. In this study, we aimed to determine the effects of the secretome of lung epithelial cells exposed to CSE on lung microvascular endothelial cells. Human lung epithelial cells, A549, were exposed to CSE, and the secretome was collected. Human lung microvascular endothelial cells, HULEC-5a, were used to evaluate the effect of the secretome of A549 exposed to CSE. Secretome profile, endothelial cell death, inflammation, and permeability markers were determined. CSE altered the secretome expression of A549 cells, and secretome derived from CSE-exposed A549 cells caused respiratory endothelial cell death, inflammation, and moderately enhanced endothelial permeability. This study demonstrates the potential role of cellular interaction between endothelial and epithelial cells during exposure to CSE and provides novel therapeutic targets or beneficial biomarkers using secretome analysis for CSE-related respiratory diseases.
Nicotiana, Pulmonary and Respiratory Medicine, Cell biology, Mechanisms and Implications of Ferroptosis in Cancer, Proteome, Secretome profiling, Science, Immunology, A549 cell, Biochemistry, Article, Endothelial stem cell, Endocrinology, In vitro, Smoke, Biochemistry, Genetics and Molecular Biology, Health Sciences, Pathology, Humans, Heme Oxygenase and Carbon Monoxide Research, Endothelium, Lung epithelial cell secretome, Vascular permeability, Molecular Biology, Lung, Biology, Internal medicine, Immunology and Microbiology, Inflammation, Macrophage Activation and Polarization, FOS: Clinical medicine, Q, R, Cigarette smoke, Endothelial Cells, Life Sciences, Epithelial Cells, Lung microvascular endothelial cell, A549 Cells, Cytoprotection, Medicine, Pulmonary disease
Nicotiana, Pulmonary and Respiratory Medicine, Cell biology, Mechanisms and Implications of Ferroptosis in Cancer, Proteome, Secretome profiling, Science, Immunology, A549 cell, Biochemistry, Article, Endothelial stem cell, Endocrinology, In vitro, Smoke, Biochemistry, Genetics and Molecular Biology, Health Sciences, Pathology, Humans, Heme Oxygenase and Carbon Monoxide Research, Endothelium, Lung epithelial cell secretome, Vascular permeability, Molecular Biology, Lung, Biology, Internal medicine, Immunology and Microbiology, Inflammation, Macrophage Activation and Polarization, FOS: Clinical medicine, Q, R, Cigarette smoke, Endothelial Cells, Life Sciences, Epithelial Cells, Lung microvascular endothelial cell, A549 Cells, Cytoprotection, Medicine, Pulmonary disease
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