Pyrosequencing Analysis Reveals Changes in Intestinal Microbiota of Healthy Adults Who Received a Daily Dose of Immunomodulatory Probiotic Strains
Copyright policy )Pyrosequencing Analysis Reveals Changes in Intestinal Microbiota of Healthy Adults Who Received a Daily Dose of Immunomodulatory Probiotic Strains
The colon microbiota plays a crucial role in human gastrointestinal health. Current attempts to manipulate the colon microbiota composition are aimed at finding remedies for various diseases. We have recently described the immunomodulatory effects of three probiotic strains (Lactobacillus rhamnosus CNCM I-4036, Lactobacillus paracasei CNCM I-4034, and Bifidobacterium breve CNCM I-4035). The goal of the present study was to analyze the compositions of the fecal microbiota of healthy adults who received one of these strains using high-throughput 16S ribosomal RNA gene sequencing. Bacteroides was the most abundant genus in the groups that received L. rhamnosus CNCM I-4036 or L. paracasei CNCM I-4034. The Shannon indices were significantly increased in these two groups. Our results also revealed a significant increase in the Lactobacillus genus after the intervention with L. rhamnosus CNCM I-4036. The initially different colon microbiota became homogeneous in the subjects who received L. rhamnosus CNCM I-4036. While some orders that were initially present disappeared after the administration of L. rhamnosus CNCM I-4036, other orders, such as Sphingobacteriales, Nitrospirales, Desulfobacterales, Thiotrichales, and Synergistetes, were detected after the intervention. In summary, our results show that the intake of these three bacterial strains induced changes in the colon microbiota.
Male, Medical Subject Headings::Anatomy::Digestive System::Gastrointestinal Tract::Intestines, Medical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::RNA::RNA, Ribosomal::RNA, Ribosomal, 16S, Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans, Feces, Medical Subject Headings::Phenomena and Processes::Microbiological Phenomena::Microbiota::Gastrointestinal Microbiome, healthy adults, Medical Subject Headings::Named Groups::Persons::Age Groups::Adult, RNA, Ribosomal, 16S, Gut, high-throughput nucleotide sequencing, Medical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::DNA::DNA, Bacterial, Medical Subject Headings::Technology, Industry, Agriculture::Food and Beverages::Food::Dietary Supplements::Probiotics, Phylogeny, Medical Subject Headings::Anatomy::Fluids and Secretions::Feces, Lacticaseibacillus rhamnosus, Microbiota, High-Throughput Nucleotide Sequencing, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Sequence Analysis::Sequence Analysis, DNA, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Sequence Analysis::High-Throughput Nucleotide Sequencing, Healthy Volunteers, Medical Subject Headings::Check Tags::Male, Intestines, Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Phylogeny, gut, Female, Adult, DNA, Bacterial, Medical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Actinobacteria::Bifidobacterium, Article, Young Adult, microbiota, Humans, Medical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Gram-Positive Rods::Gram-Positive Asporogenous Rods::Gram-Positive Asporogenous Rods, Regular::Lactobacillaceae::Lactobacillus, Probiotics, Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Young Adult, Medical Subject Headings::Check Tags::Female, Sequence Analysis, DNA, Gastrointestinal Microbiome, Lactobacillus, Healthy adults, probiotics, Medical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Gram-Positive Rods::Gram-Positive Asporogenous Rods::Gram-Positive Asporogenous Rods, Regular::Lactobacillaceae::Lactobacillus::Lactobacillus rhamnosus, Medical Subject Headings::Named Groups::Persons::Research Subjects::Healthy Volunteers, Bifidobacterium, High-throughput nucleotide sequencing
Male, Medical Subject Headings::Anatomy::Digestive System::Gastrointestinal Tract::Intestines, Medical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::RNA::RNA, Ribosomal::RNA, Ribosomal, 16S, Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans, Feces, Medical Subject Headings::Phenomena and Processes::Microbiological Phenomena::Microbiota::Gastrointestinal Microbiome, healthy adults, Medical Subject Headings::Named Groups::Persons::Age Groups::Adult, RNA, Ribosomal, 16S, Gut, high-throughput nucleotide sequencing, Medical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::DNA::DNA, Bacterial, Medical Subject Headings::Technology, Industry, Agriculture::Food and Beverages::Food::Dietary Supplements::Probiotics, Phylogeny, Medical Subject Headings::Anatomy::Fluids and Secretions::Feces, Lacticaseibacillus rhamnosus, Microbiota, High-Throughput Nucleotide Sequencing, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Sequence Analysis::Sequence Analysis, DNA, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Sequence Analysis::High-Throughput Nucleotide Sequencing, Healthy Volunteers, Medical Subject Headings::Check Tags::Male, Intestines, Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Phylogeny, gut, Female, Adult, DNA, Bacterial, Medical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Actinobacteria::Bifidobacterium, Article, Young Adult, microbiota, Humans, Medical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Gram-Positive Rods::Gram-Positive Asporogenous Rods::Gram-Positive Asporogenous Rods, Regular::Lactobacillaceae::Lactobacillus, Probiotics, Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Young Adult, Medical Subject Headings::Check Tags::Female, Sequence Analysis, DNA, Gastrointestinal Microbiome, Lactobacillus, Healthy adults, probiotics, Medical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Gram-Positive Rods::Gram-Positive Asporogenous Rods::Gram-Positive Asporogenous Rods, Regular::Lactobacillaceae::Lactobacillus::Lactobacillus rhamnosus, Medical Subject Headings::Named Groups::Persons::Research Subjects::Healthy Volunteers, Bifidobacterium, High-throughput nucleotide sequencing
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