Lysosomal exoglycosidases in serum and urine of patients with pancreatic adenocarcinoma.
Copyright policy )Lysosomal exoglycosidases in serum and urine of patients with pancreatic adenocarcinoma.
Lysosomal exoglycosidases: N-acetyl-β-D-hexosaminidase (HEX), β-D-galactosidase (GAL), α-L-fucosidase (FUC) and α-D-mannosidase (MAN) modify oligosaccharide chains of glycoconjugates in endoplasmatic reticulum and/or Golgi apparatus and degrade them in lysosomes. In acid environment of lysosome, exoglycosidases degrade oligosaccharide chains of glycoproteins, glycolipids and glycosaminoglycans by eliminating single sugars from the edges of oligosaccharide chains. Neoplasms change biochemical processes in tissues and may significantly change the activity of many enzymes including the activity of lysosomal exoglycosidasses in serum and urine of persons with neoplasmatic diseases. The aim of the present paper was evaluation the activity of HEX, GAL, FUC and MAN in serum and urine of patients with pancreatic adenocarcinoma. Serum and urine samples were collected from 15 patients with adenocarcinoma of the pancreas and 15 healthy persons. The activity of lysosomal exoglycosidases was determined by the method of Marciniak et al. adapted to serum and urine of patients with adenocarcinoma of the pancreas. Our results indicate significant decrease in activity of GAL (p=0.037) in serum of patients with pancreatic adenocarcinoma, significant increase in activity of HEX (p<0.001) and FUC (p=0.027) in serum, and HEX (p=0.003) in urine, as well as significant decrease of FUC (p=0.016) and MAN (p=0.029) in urine o patients with adenocarcinoma of the pancreas, in comparison to the control group. Increase in activity of some lysosomal enzymes in serum and urine of pancreatic adenocarcinoma patients, may indicate on destruction of pancreatic tissue by pancreatic adenocarcinoma. Determination of the HEX, GAL, FUC and MAN in serum and urine may be useful in diagnostics of pancreatic adenocarcinoma.
- Medical University of Lodz Poland
- Wrocław Medical University Poland
- Medical University of Białystok Poland
Male, Serum, Glycoconjugates - urine, beta-Galactosidase - urine, Beta-N-Acetylhexosaminidases - metabolism, Serum - enzymology, Pancreatic neoplasms - urine, Adenocarcinoma - enzymology, Adenocarcinoma - urine, Middle aged, beta-Galactosidase - metabolism, alpha-L-Fucosidase, Lysosomes - metabolism, Middle Aged, Alpha-Mannosidase - metabolism, beta-N-Acetylhexosaminidases, Sensitivity and specificity, Female, Glycoconjugates - metabolism, Adult, Glycoside Hydrolases, Beta-N-Acetylhexosaminidases - urine, Case-control studies, Adenocarcinoma, Lysosomes - enzymology, Glycoside hydrolases - blood, alpha-L-fucosidase - metabolism, Sensitivity and Specificity, alpha-Mannosidase, Adenocarcinoma - blood, Glycoside hydrolases - urine, Humans, alpha-L-fucosidase - urine, Aged, Pancreatic neoplasms - blood, QH573-671, Beta-N-Acetylhexosaminidases - blood, beta-Galactosidase, Pancreatic Neoplasms, Case-Control Studies, Serum - metabolism, Cytology, Lysosomes, Pancreatic neoplasms - enzymology, Glycoconjugates
Male, Serum, Glycoconjugates - urine, beta-Galactosidase - urine, Beta-N-Acetylhexosaminidases - metabolism, Serum - enzymology, Pancreatic neoplasms - urine, Adenocarcinoma - enzymology, Adenocarcinoma - urine, Middle aged, beta-Galactosidase - metabolism, alpha-L-Fucosidase, Lysosomes - metabolism, Middle Aged, Alpha-Mannosidase - metabolism, beta-N-Acetylhexosaminidases, Sensitivity and specificity, Female, Glycoconjugates - metabolism, Adult, Glycoside Hydrolases, Beta-N-Acetylhexosaminidases - urine, Case-control studies, Adenocarcinoma, Lysosomes - enzymology, Glycoside hydrolases - blood, alpha-L-fucosidase - metabolism, Sensitivity and Specificity, alpha-Mannosidase, Adenocarcinoma - blood, Glycoside hydrolases - urine, Humans, alpha-L-fucosidase - urine, Aged, Pancreatic neoplasms - blood, QH573-671, Beta-N-Acetylhexosaminidases - blood, beta-Galactosidase, Pancreatic Neoplasms, Case-Control Studies, Serum - metabolism, Cytology, Lysosomes, Pancreatic neoplasms - enzymology, Glycoconjugates
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