Purification and characterization of a new β-glucosidase from Penicillium piceum and its application in enzymatic degradation of delignified corn stover
Copyright policy )Purification and characterization of a new β-glucosidase from Penicillium piceum and its application in enzymatic degradation of delignified corn stover
A new β-glucosidase (Cel3B) was first isolated from cellulytic fungi, designated as PpCel3B. Although PpCel3B was classified to GH family 3 based on the homology sequence, PpCel3B had different biological functions in cellulose degradation and signaling molecules production. PpCel3B was constitutive and could form multiple soluble lignocellulose inducers for cellulase and hemicellulase synthesis via high tranglycosylation activity and new enzymatic activity. Moreover, PpCel3B showed apparent synergism with cellulases by removing several inhibitors. Supplementing low doses of PpCel3B (52 μg/g substrate) increased saccharification efficiency of cellulase produced by Trichoderma reesei and Penicillium piceum by 15% and 35%, respectively on delignified corn stover. PpCel3B had important application in boosting cellulase yield and efficiency.
- Washington State University United States
- Chinese Academy of Sciences, Tianjin Institute of Industrial Biotechnology China (People's Republic of)
- Chinese Academy of Sciences China (People's Republic of)
- Chinese Academy of Sciences (中国科学院) China (People's Republic of)
- Chinese Academy of Science (中国科学院) China (People's Republic of)
Electrophoresis, 570, β-1,4-Glucosidase, Environmental Engineering, Bioengineering, Lignin - metabolism, beta-Glucosidase - metabolism, Lignin, Zea mays, 630, Substrate Specificity, PpCel3B, Cellulase, Matrix-Assisted Laser Desorption-Ionization, Waste Management and Disposal, Zea mays - metabolism, DNA Primers, Polyacrylamide Gel, Base Sequence, Spectrometry, Reverse Transcriptase Polymerase Chain Reaction, beta-Glucosidase, Synergism, Penicillium, Mass, Cellulase - metabolism, Penicillium - enzymology, Inducer, beta-Glucosidase - isolation & purification, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Electrophoresis, Polyacrylamide Gel
Electrophoresis, 570, β-1,4-Glucosidase, Environmental Engineering, Bioengineering, Lignin - metabolism, beta-Glucosidase - metabolism, Lignin, Zea mays, 630, Substrate Specificity, PpCel3B, Cellulase, Matrix-Assisted Laser Desorption-Ionization, Waste Management and Disposal, Zea mays - metabolism, DNA Primers, Polyacrylamide Gel, Base Sequence, Spectrometry, Reverse Transcriptase Polymerase Chain Reaction, beta-Glucosidase, Synergism, Penicillium, Mass, Cellulase - metabolism, Penicillium - enzymology, Inducer, beta-Glucosidase - isolation & purification, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Electrophoresis, Polyacrylamide Gel
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