Improvement of Oligonucleotide Probe Design Criteria for Functional Gene Microarrays in Environmental Applications
Copyright policy )Improvement of Oligonucleotide Probe Design Criteria for Functional Gene Microarrays in Environmental Applications
ABSTRACT To optimize oligonucleotide probe design criteria, PCR products with different similarities to probes were hybridized to a functional gene microarray designed to detect homologous genes from different organisms. In contrast to more restrictive probe designs based on a single criterion, simultaneous consideration of the percent similarity (≤90%), the length of identical sequence stretches (≤20 bases), and the binding free energy (≥−35 kcal mol −1 ) was found to be predictive of probe specificity.
- Chungbuk National University Korea (Republic of)
- Helmholtz Association of German Research Centres Germany
- Forschungszentrum Jülich Germany
- Oak Ridge National Laboratory United States
info:eu-repo/classification/ddc/570, Oligonucleotide Probes: genetics, Base Sequence, Oligonucleotide Probes: chemistry, Nucleic Acid Hybridization, Polymerase Chain Reaction, J, Drug Design, Environmental Microbiology, Thermodynamics, Oligonucleotide Probes, Oligonucleotide Array Sequence Analysis: statistics & numerical data, Oligonucleotide Array Sequence Analysis: methods, Oligonucleotide Array Sequence Analysis
info:eu-repo/classification/ddc/570, Oligonucleotide Probes: genetics, Base Sequence, Oligonucleotide Probes: chemistry, Nucleic Acid Hybridization, Polymerase Chain Reaction, J, Drug Design, Environmental Microbiology, Thermodynamics, Oligonucleotide Probes, Oligonucleotide Array Sequence Analysis: statistics & numerical data, Oligonucleotide Array Sequence Analysis: methods, Oligonucleotide Array Sequence Analysis
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