Internal fit is an important aspect of indirect restorations, but methods for the 3-dimensional (3D) measurement of absolute marginal and intaglio fit are sparse.The purpose of this in vitro study was to evaluate an innovative 3D measurement method (AIXFit) based on intraoral scanning data for analyzing the fit of dental restorations.For the evaluation of AIXFit, 12 monolithic zirconia crowns were fabricated on typodont preparations. The fit was measured digitally with the AIXFit system and compared with the results obtained from an established 2-dimensional (2D) sectional procedure. To compare the values of both methods at identical locations, a common reference system was developed, with each die fixed in a gypsum stand with reference points. Using an intraoral scanner (True Definition), each die with its reference points and the intaglio surface of the finished crown were digitalized as standard tessellation language files. The AIXFit software program, with a specially developed best-fit algorithm, was used to match the intaglio surface of the crown with the surface of the preparation. The virtual cement gap was calculated over the entire surface and returned values for x≥0 µm. A 2D comparison method involved adhesively fixing the crown to the die and sectioning it into 4 parts with a diamond band saw. The thickness of the cement gap was determined under a light microscope at ×100 magnification at 5 defined measuring points per quarter, so that a total of 240 measurements were available for comparison. A software program (Blender Foundation) was used to superimpose the data from the AIXFit system with the data from the 2D method and to compare the cement gaps at the same locations. The agreement between these methods was verified using paired t tests and determine correlation coefficients (α=.05).The mean ±standard deviation difference between the AIXFit and 2D methods was 6.7 ±29 µm). Two 1-sided tests showed statistical equivalence between the methods of measurement when considering an interval between -20 and +20 µm. The correlation coefficients showed a positive association for both methods (r=.931).The AIXFit software program appeared to be accurate for the digital measurement of internal fit when using the True Definition scanner. It enabled a cast-free workflow and allowed the analysis of the entire intaglio surface.