Objectives Studies on accelerometer-measured daily physical activity behaviour, especially among hybrid and remote workers, are scarce. We compared daily occupational and non-occupational physical activity and sedentary time among manual, in-office, hybrid and remote workers. In addition, physical activity behaviour during remote and office workdays among hybrid workers was compared. Methods Daily physical activity behaviour was collected with wrist-worn accelerometers on ≥4 days from 133 Finnish workers (31 years, 61% women). Participants were divided into four groups according to their work modes: manual (n=32), in-office (n=49), hybrid (n=35) and remote workers (n=17). Differences in physical activity and sedentary time during workdays (separately for occupational and non-occupational time) between the groups were examined using generalised linear models. Linear mixed models were used for intra-individual differences among hybrid workers. Results Workdays’ occupational physical activity and sedentary time differed between the work mode groups (p<0.0001); the manual workers accumulated the highest occupational physical activity, while both hybrid and remote workers accumulated the highest occupational sedentary time. No differences in non-occupational behaviours were observed. Among hybrid workers, occupational sedentary time tended to be higher (26 min, 95% CI −2 to 53) during remote versus office workdays, but non-occupational behaviours were similar. Conclusions Remote work is associated with the lowest physical activity and the highest sedentary time compared with other work modes. Strategies to promote physical activity during remote workdays may be needed.