The purpose of this article is an evaluation of the impact of changes in regulations concerning income tax assessment and premiums for medical insurance from economic activity of microenterprises as part of the so-called „Polish Deal” on the value of regulatory liabilities arising from income tax and health contribution in context of various forms of taxations. Ambiguity and changeability of the regulations as well as an increase in regulatory liabilities is not helpful to entrepreneurs in business development and constitutes obstacles, hindering the advancement of the microenterprise sector. Choosing the taxation form of conducted business is very significant for the amount of income tax expense of microenterprise. Amendments to tax regulations due to the 2022 introduction of the „Polish Deal” caused a revolution in forms of accounting for income tax and regulations defining the value of premiums for medical insurance in microenterprise groups. It results from the conducted research that implementing regulations of the „Polish Deal” caused the increase of the regulatory liabilities for microenterprises which in turn in some cases result in a drop in profitability of conducted activity that can hinder entrepreneurship and lead to abandonment of conducting economic activity.