Downloads provided by UsageCountsRelationship between Proinflammatory and Antioxidant Proteins with the Severity of Cardiovascular Disease in Type 2 Diabetes Mellitus
Copyright policy ) García-Fontana, Beatriz; Morales-Santana, Sonia; Longobardo, Victoria; Reyes-García, Rebeca; Rozas-Moreno, Pedro; García-Salcedo, José Antonio; Muñoz-Torres, Manuel;
Relationship between Proinflammatory and Antioxidant Proteins with the Severity of Cardiovascular Disease in Type 2 Diabetes Mellitus
Type 2 diabetes mellitus patients are at significant risk of cardiovascular disease, however, the pathophysiology of these complications is complex and incompletely known in this population. The aim of this study was to compare the serum proteome of patients with type 2 diabetes mellitus presenting or not presenting cardiovascular disease with non-diabetic subjects to find essential proteins related to these cardiovascular complications. This cross-sectional study compares the serum proteome by a combination of protein depletion with 2D-DIGE (2-dimension Difference Gel Electrophoresis) methodology. The proteins differentially expressed were identified by MALDI TOF/TOF (Matrix-assisted laser desorption/ionization and Time-Of-Flight ion detector) or LC-MS/MS (Liquid Chromatography coupled to Mass-Mass Spectrometry). Type 2 diabetes mellitus patients with cardiovascular disease showed higher expression of plasma retinol binding protein and glutathione peroxidase-3 compared to those without cardiovascular disease and non-diabetic controls. These results show that proteins related to the inflammatory and redox state appear to play an important role in the pathogenesis of the cardiovascular disease in the type 2 diabetes mellitus patients.
- Instituto de Investigación Biosanitaria Spain
- Yahoo! United States
- Spanish National Research Council Spain
- Institute of Parasitology and Biomedicine "López-Neyra" Spain
Medical Subject Headings::Diseases::Endocrine System Diseases::Diabetes Mellitus::Diabetes Mellitus, Type 2, Male, Proteomics, Medical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Molecular Mechanisms of Pharmacological Action::Antioxidants, type 2 diabetes mellitus, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Mass Spectrometry::Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Antioxidants, Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans, Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Oxidoreductases::Peroxidases::Glutathione Peroxidase, cardiovascular disease, Medical Subject Headings::Named Groups::Persons::Age Groups::Adult, oxidative stress, Electrophoresis, Gel, Two-Dimensional, Proinflammation, Medical Subject Headings::Diseases::Endocrine System Diseases::Diabetes Mellitus::Diabetes Complications, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Prognosis, proteomic, Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Middle Aged, Medical Subject Headings::Diseases::Cardiovascular Diseases, Anthropometry, Medical Subject Headings::Diseases::Pathological Conditions, Signs and Symptoms::Pathologic Processes::Inflammation, Middle Aged, Cardiovascular disease, Prognosis, proinflammation, Medical Subject Headings::Check Tags::Male, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Electrophoresis::Electrophoresis, Gel, Two-Dimensional::Two-Dimensional Difference Gel Electrophoresis, Cardiovascular Diseases, Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Chemical Processes::Physicochemical Processes::Oxidation-Reduction, Oxidation-Reduction, Adult, Medical Subject Headings::Chemicals and Drugs::Biological Factors::Biomarkers, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Cross-Sectional Studies, Article, Diabetes Complications, Medical Subject Headings::Phenomena and Processes::Metabolic Phenomena::Metabolism::Oxidative Stress, Medical Subject Headings::Disciplines and Occupations::Natural Science Disciplines::Biological Science Disciplines::Biochemistry::Proteomics, Type 2 diabetes mellitus, Humans, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Data Collection::Health Surveys::Health Status Indicators::Severity of Illness Index, Inflammation, Glutathione Peroxidase, Proteomic, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Physical Examination::Anthropometry, Retinol-Binding Proteins, Cellular, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Mass Spectrometry::Tandem Mass Spectrometry, Oxidative Stress, Cross-Sectional Studies, Diabetes Mellitus, Type 2, Oxidative stress, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Chromatography::Chromatography, Liquid, Biomarkers, Chromatography, Liquid
Medical Subject Headings::Diseases::Endocrine System Diseases::Diabetes Mellitus::Diabetes Mellitus, Type 2, Male, Proteomics, Medical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Molecular Mechanisms of Pharmacological Action::Antioxidants, type 2 diabetes mellitus, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Mass Spectrometry::Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Antioxidants, Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans, Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Oxidoreductases::Peroxidases::Glutathione Peroxidase, cardiovascular disease, Medical Subject Headings::Named Groups::Persons::Age Groups::Adult, oxidative stress, Electrophoresis, Gel, Two-Dimensional, Proinflammation, Medical Subject Headings::Diseases::Endocrine System Diseases::Diabetes Mellitus::Diabetes Complications, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Prognosis, proteomic, Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Middle Aged, Medical Subject Headings::Diseases::Cardiovascular Diseases, Anthropometry, Medical Subject Headings::Diseases::Pathological Conditions, Signs and Symptoms::Pathologic Processes::Inflammation, Middle Aged, Cardiovascular disease, Prognosis, proinflammation, Medical Subject Headings::Check Tags::Male, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Electrophoresis::Electrophoresis, Gel, Two-Dimensional::Two-Dimensional Difference Gel Electrophoresis, Cardiovascular Diseases, Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Chemical Processes::Physicochemical Processes::Oxidation-Reduction, Oxidation-Reduction, Adult, Medical Subject Headings::Chemicals and Drugs::Biological Factors::Biomarkers, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Cross-Sectional Studies, Article, Diabetes Complications, Medical Subject Headings::Phenomena and Processes::Metabolic Phenomena::Metabolism::Oxidative Stress, Medical Subject Headings::Disciplines and Occupations::Natural Science Disciplines::Biological Science Disciplines::Biochemistry::Proteomics, Type 2 diabetes mellitus, Humans, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Data Collection::Health Surveys::Health Status Indicators::Severity of Illness Index, Inflammation, Glutathione Peroxidase, Proteomic, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Physical Examination::Anthropometry, Retinol-Binding Proteins, Cellular, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Mass Spectrometry::Tandem Mass Spectrometry, Oxidative Stress, Cross-Sectional Studies, Diabetes Mellitus, Type 2, Oxidative stress, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Chromatography::Chromatography, Liquid, Biomarkers, Chromatography, Liquid
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