Bacterial cellulose (BC) is widely used in medicine as a dressing material due to its good biological properties – high biocompatibility, low adhesion and the ability to absorb wound exudate. The BC does not have antimicrobial activity itself, which limits the use of products in infected wounds, and also treatment of wounds in hospitals. A method for producing a two-component composite material based on bacterial cellulose (BC) synthesized in culture of Komagataeibacter xylinus B-12068 with silver nanoparticles [BC/AgNps], by hydrothermal synthesis of AgNO3 in the layer of BC at different temperatures and concentrations of AgNO3, is proposed. The antibacterial activity of BC/AgNps samples against Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, and Staphylococcus aureus has been confirmed in vitro with the disc diffusion method. It is shown, that the antibacterial activity of samples is mostly expressed in cultures of P. aeruginosa and S. aureus