The article is devoted to the influence of some microelements on the growth and activity of antioxidant oxidoreductase of basidiomycetes. The objects of the study were strains – active producers of oxidoreductases: Agrocybe cylindracea 167; Pleurotus ostreatus P-208 and Fistulina hepatica Fh-08. The weighting method of determination of accumulation of absolutely dry biomass was used to study the influence of some microelements on the growth. Catalase, peroxidase and superoxide dismutase activity and protein content of mycelium and culture filtrate was determined by spectrophotometric methods, and the specific activity of enzymes were calculated. A significant effect of Fe2+, Cu2+, Zn2+ and Mn2+ on the level of accumulation of absolutely dry biomass, catalase, peroxidase and superoxide dismutase activity was estimated. It was found that the stimulation of peroxidase activity in mycelia and culture filtrate of the strains P. ostreatus P-208 and F. hepatica Fh-08 caused by the addition of Fe2+ in the concentration of 8 and 1.6 mmol / l, and the stimulation of their catalase activity caused by the addition of Cu2+ and Mn2+ in the concentration of 8 mmol / l. Increased catalase activity of strain A. cylindracea is 167 caused by amending Cu2+ and Zn2+ in the concentration of 8 mmol / l in the medium. There was a slight stimulation of superoxide dismutase activity of the strains of basidiomycetes caused by addition of ferrous sulphate in the nutrient medium. The results of the study showed the relationship between the composition of culture media and the structure, function and localization of enzymes and their interaction.