One-dimensional photonic crystals have been used in sensing applications for decades, due to their ability to induce highly reflective photonic bandgaps. In this study, one-dimensional photonic crystals with alternating low- and high-density layers were fabricated from a single photosensitive polymer (IP-Dip) by two-photon polymerization. The photonic crystals were modified to include a central defect layer with different elastic properties compared to the surrounding layers, for the first time. It was observed that the defect mode resonance can be controlled by compressive force. Very good agreement was found between the experimentally measured spectra and the model data. The mechanical properties of the flexure design used in the defect layer were calculated. The calculated spring constant is of similar magnitude to those reported for microsprings fabricated on this scale using two-photon polymerization. The results of this study demonstrate the successful control of a defect resonance in one-dimensional photonic crystals fabricated by two-photon polymerization by mechanical stimuli, for the first time. Such a structure could have applications in fields, such as micro-robotics, and in micro-opto–electro–mechanical systems (MOEMSs), where optical sensing of mechanical fluctuations is desired.