The world’s leading health institutions warn that adolescents do not meet the minimum level of physical activity necessary for the proper physical development of young people, which is considered one of the main problems of public health. In addition to personal preference, environmental and cultural factors play an important role in the involvement of young people in sports. This study aimed to examine potential differences in involvement in sports, anthropometric characteristics and motor abilities, in adolescents of different residential statuses. The sample included 83 adolescents, of whom 45 (54.2%, 13.51±0.55 yrs) were from urban areas and 38 (45%, 13.45±0.50 yrs) were from rural areas in the Belgrade region. Surveying detected their involvement in sports, and anthropometric characteristics were assessed through body height (BH) body mass (BM), body mass index (BMI), and motor abilities through explosive strength of lower and upper extremities and agility. A Mann-Whitney U test has shown that place of residency impacts involvement in sports and motor abilities, while there was no impact on anthropometric characteristics. Cohen’s criteria (r) detected the level of impact. Adolescents from urban areas are more involved at sports and have better motor abilities. There is no difference in anthropometric characteristics. Considering the relatively small sample of respondents and variables assessed, the obtained results can hardly be generalized. However, this does not diminish the importance of the present study which examined the urban-rural state of sports activity, anthropometric characteristics and motor abilities of adolescents in Serbia, thus making an important contribution to this field.